牛结节性皮肤病病毒感染MDBK细胞后转录组差异表达分析

doi:10.11843/j.issn.0366-6964.2021.02.018

Abstract

Abstract: To explore the interaction between LSDV and host cells, in this study, cattle skin samples with typical lesions of LSD was collected, and MDBK cells were inoculated for virus isolation. Skin lesions and infected MDBK cells were observed by transmission electron microscopy. Besides, transcriptomics analysis was performed on MDBK cells 24 hours after infection to screen differentially expressed genes, classify them according to their functions, and analyze the cell signaling pathways involving them. The results showed that LSDV was elliptic under electron microscope and the virion size was about 320 nm×260 nm. Compared with the untreated group, a total of 499 genes were significantly differentially expressed, among which 112 genes were up-regulated and 387 genes were down-regulated through transcriptome analysis. According to GO and KEGG analysis, the differentially expressed genes were mainly concentrated in the MAPK signaling pathway that transmits signals from the cell surface to the inside of the nucleus, in which the expression of MAP3K1 gene, which is involved in innate immunity and associated in apoptosis, showed significant downregulation, suggesting that this viral infection may affect the migration and apoptosis of host cells. The significantly changed genes include HIF1A, RORA, LRRK2, AP3B1 and ANO6, which are respectively involved in functions such as regulating vascular endothelial growth factor (VEGF), inflammatory response, proliferation and differentiation of cells and signal transduction. This study laid a foundation for further exploring the pathogenic mechanism of LSDV.

Key words: lumpy skin disease virus (LSDV), MDBK cells, high throughput sequencing, transcriptome

CLC Number:

S852.659.1

CAO Zheng, JUMABIEKE Xialabayi, LI Chunyan, MALIKE Aizezi. Transcriptome Analysis of MDBK Cells Infected with Lumpy Skin Disease Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(2): 460-468.

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Transcriptome Analysis of MDBK Cells Infected with Lumpy Skin Disease Virus

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