己糖激酶2在犬乳腺肿瘤中的表达及预后研究

doi:10.11843/j.issn.0366-6964.2023.03.041

Abstract

Abstract: This experiment was conducted to study the expression of hexokinase 2 (HK2) in canine mammary tumor (CMT) and its correlation with the prognosis of dogs. Canine mammary masses from 121 clinical samples were made into paraffin sections. After histopathological analysis and interpretation, 3 samples of malignant mammary tumors and 3 samples of normal mammary gland tissues were randomly selected for high-throughput transcriptome sequencing to screen differentially expressed genes. Quantitative real time PCR (qPCR) and immunohistochemistry were applied to detect the expression of HK2 in the overall body tissues and mammary tumors, and long-term follow-up of dogs was conducted to investigate and analyze the correlation between HK2 and the clinical characteristics and prognosis of dogs with mammary tumor. The results showed that: 1) There were 33 benign and 78 malignant mammary tumors in 121 clinical samples of canine mammary masses collected in this study; 2) Thirty-six significantly and differentially expressed genes such as HK2, MEST, GDF5 and etc, were screened out by high-throughput transcriptome sequencing. Based on the verification by qPCR and the key role played in cell metabolism, and abnormal expression in some human tumors. HK2 was designated as the research object; 3) The expression levels of HK2 mRNA and protein were low in normal canine mammary gland tissues, but significantly elevated in CMT (P<0.01); 4) The expression level of HK2 was significantly correlated with tumor tissue type, tumor size, vascular invasion, Ki-67 index and prognosis (P<0.01). HK2 is highly expressed in CMT tissues, and is significantly related to the clinical characteristics and prognosis of CMT. HK2 can be potentially regarded as a diagnostic and prognostic factor of CMT.

Key words: canine mammary tumor, hexokinase 2, glucose metabolism, high throughput sequencing

CLC Number:

XU Huihao, LIU Jiangyu, LI Qijuan, ZHENG Xiaobo, LIN Jiahao, JIN Yipeng, LIN Degui. Expression and Prognosis of Hexokinase 2 in Canine Mammary Tumors[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1310-1324.

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Expression and Prognosis of Hexokinase 2 in Canine Mammary Tumors

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