基于多组学与网络药理学探究淫羊藿对后备母猪发情的作用

doi:10.11843/j.issn.0366-6964.2024.04.024

Abstract

Abstract: The aim of this study was to reveal the effect and mechanism of epimedium on the estrus of gilts. A total of 32 gilts aged 210-220 days old, weighing (99.547±1.987)kg, mature and meeting breeding conditions were selected for the experiment. They were randomly divided into a control group and an experimental group, with 16 sows in each group and one sow in each replicate. Individuals in the control group was fed with a basic diet, while individuals in the experimental group was supplemented with 50 mg·d^-1 crude extract of epimedium in addition to the basic diet. Experimental feeding for 28 days. The results showed that gilts in the experimental group experienced early estrus, and serum FSH, LH, and E₂ significantly increased (P < 0.05). The ovarian transcriptome results showed that 477 upregulated differential mRNAs and 754 downregulated differential mRNAs were detected. GO enrichment analysis revealed that differential mRNAs was mainly enriched in processes such as transport vesicles, adipocyte differentiation, rhythmic behavior, and estrus cycle; KEGG enrichment analysis revealed that differential mRNAs was mainly enriched in tight junctions, carbohydrate metabolism, hedgehog signaling pathways, GnRH secretion, and PI3K-AKT signaling pathways. The results of ovarian metabolomics showed a total of 1 616 metabolites upregulated and 1 254 metabolites downregulated. KEGG enrichment analysis showed that differential metabolites were mainly enriched in α-linoleic acid metabolism, ATP transporters, linoleic acid metabolism β-pathways such as alanine metabolism, aminobenzoate degradation, and biotin metabolism. The results of network pharmacology analysis indicated that there were a total of 161 potential targets for the action of epimedium on the ovaries. The PPI interaction network obtained the top 10 protein grades as core targets, which were TP53, SRC, AKT1, CCND1, TNF, ESR1, EP300, ERBB2, JAK2, and PARP1 according to their scores. The GO analysis results indicated that the targets of epimedium on the ovaries were mainly involved in biological processes such as protein phosphorylation, positive regulation of MAPK cascade reaction, biological rhythm, positive regulation of gene expression, negative regulation of cell apoptosis process, positive regulation of intracellular calcium ion concentration, and positive regulation of RNA polymerase II promoter transcription. The KEGG analysis results showed that the target protein signaling pathway was enriched in PI3K-AKT signaling pathway, cell cycle, cell aging, HIF-1 signaling pathway, progesterone mediated oocyte maturation, and other pathways. The above results reveal the effects and pathways of epimedium on the estrus of gilts from the perspectives of in vivo, metabolism, transcription, and molecular level. This study found that feeding epimedium can change the transcription and metabolism patterns of sows′ ovaries, significantly increase FSH, LH, and E₂ levels, and promote estrus in gilts. The main components of epimedium can bind with TP53, SRC, AKT1, CCND1, TNF, ESR1, EP300, ERBB2, JAK2, and PARP1 to exert regulatory effects. This study provides a theoretical basis for the application of epimedium to improve the utilization rate of estrus in gilts.

Key words: epimedium, gilts, estrus, transcriptome, metabolic group, network pharmacology

CLC Number:

S828.3

XU Junjie, ZHANG Lutong, WANG Jinjie, CHEN Xiaochen, HE Weixian, CAI Chuanjiang, CHU Guiyan, YANG Gongshe. Exploring the Effect of Epimedium on Estrus of Gilts Based on Multiomics and Network Pharmacology[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1615-1628.

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Exploring the Effect of Epimedium on Estrus of Gilts Based on Multiomics and Network Pharmacology

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