基于转录组学研究日粮添加尿素对育肥湖羊肝组织氨代谢的影响

doi:10.11843/j.issn.0366-6964.2023.03.026

Abstract

Abstract: The aim of this study was to evaluate the effects of dietary urea supplementation with different levels on liver ammonia metabolism and related metabolic pathways by transcriptomic method in fattening Hu lambs. Forty-two 3-month-old fattening male Hu lambs with similar body weight of (24.3±1.7) kg were selected and randomly assigned to one of three experimental diets, which supplemented with 0 (U0 group), 10 (U10 group) and 30 (U30 group) g·kg^-1 urea on the basis of basal diets, respectively. The experiment consisted of 1 week of adaptation followed by 10 weeks of dietary treatments. At the end of the feeding trial, six animals from each group were harvested. Liver tissues of each lamb were collected to measure the concentration of ammonia-N and urea-N, and the RNA was extracted for liver transcriptome analysis. The differentially expressed genes (DEGs) were identified based on |fold change|>2 and P<0.05, and gene ontology (GO) analysis and kyoto encyclopedia of genes and genomes (KEGG) annotation were conducted. The results showed that urea supplementation increased the ammonia-N concentration in liver, and the ammonia-N concentration in U10 group was significantly higher than that in U0 group (P<0.05). Compared with U0 and U10 groups, the hepatic urea-N concentration was greatly increased in U30 group (P<0.05). Transcriptome sequencing results showed that a total of 546 DEGs were obtained by pairwise comparison among all groups. Compared with U0 group, U10 and U30 group both had 85 up-regulated DEGs, and 95 and 108 down-regulated DEGs, respectively. Compared with U10 group, 58 up-regulated DEGs and 115 down-regulated DEGs were found in U30 group. GO functional annotation results suggested that these DEGs were mainly annotated in GO terms, including amine metabolism and catabolism, regulation of immune system, and so on. Compared with U0 group, KEGG enrichment analysis showed that the energy metabolic pathway of U10 group were mainly enriched in lipid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, xenobiotics biodegradation and metabolism, and most of its genes were significantly up-regulated. While with the urea addition to the diet further increased, the metabolic pathway of U30 group were mainly associated with the immune system, including RIG-I like receptor signaling pathway, cytosolic DNA-sensing pathway and chemokine signaling pathway. Compared with U0 group, the expression of key enzyme genes such as arginase (ARG) and glutamine synthetase (GLUL) in urea biosynthesis and glutamine synthesis pathway in liver were significantly up-regulated in the groups with urea addition (P<0.05). In conclusion, dietary urea supplementation increased the concentration of urea-N in liver by up-regulating the expression of key enzyme genes in arginine biosynthesis pathway, thus affecting liver ammonia metabolism.

Key words: urea, fattening Hu lambs, liver, transcriptome

CLC Number:

S826.5

SUN Meijie, CAO Liwen, ZHENG Wenjin, SHEN Junshi, ZHU Weiyun. Effect of Dietary Urea Supplementation on Liver Ammonia Metabolism in Fattening Hu Lambs Based on Transcriptome Sequencing[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1148-1159.

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Effect of Dietary Urea Supplementation on Liver Ammonia Metabolism in Fattening Hu Lambs Based on Transcriptome Sequencing

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