gga-miR-155对MDCC-MSB1细胞转录组的影响

doi:10.11843/j.issn.0366-6964.2023.02.023

Abstract

Abstract: This study aimed to investigate the effects of gga-miR-155 on the transcriptomic level of Marek's disease virus transformed tumor cell line MDCC-MSB1 cells. In this study, MDCC-MSB1 cells were transfected with gga-miR-155 mimics and gga-miR-155 mimics NC, the total RNA of MDCC-MSB1 cells transfected with gga-miR-155 mimics and its negative control was extracted at 48 h after transfection and the integrity of total RNA was analyzed by Agilent 2100 nucleic acid detector respectively. After analyzing the overexpression of gga-miR-155 in MDCC-MSB1 cells by RT-qPCR, the changes in transcription level of MDCC-MSB1 cells after overexpression of gga-miR-155 were analyzed using the high-throughput sequencing technology. On this basis, the differentially expressed genes were screened, and the involved target mRNAs of gga-miR-155 in the differentially expressed genes were predicted combined with bioinformatics analysis. The RNA samples of gga-miR-155 overexpression in MDCC-MSB1 cells were prepared and a high-throughput sequencing library was constructed. Compared with the negative control group, 87 differentially expressed genes were found in the gga-miR-155 overexpression group, including 47 up-regulated genes and 40 down-regulated genes. GO and KEGG analysis showed that these differentially expressed genes were significantly enriched in metabolic and protein binding processes/signaling pathways. Chicken BACH1 was predicted as the target mRNA of gga-miR-155 by Targetscan and miRada software (P<0.05). The transcriptional level of MDCC-MSB1 cells was regulated by overexpressed gga-miR-155, and BACH1 gene in the differentially expressed genes may be involved in the metabolic process of MDCC-MSB1 cells under the regulation of gga-miR-155.

Key words: gga-miR-155, Marek's disease, MDCC-MSB1 cells, transcriptome

CLC Number:

S831.7

YU Zuhua, JIA Yanyan, HE Lei, LIAO Chengshui, LI Jing, WEI Ying, CHEN Jian, CHEN Songbiao, SHANG Ke, DING Ke. Effects of gga-miR-155 on MDCC-MSB1 Cell Transcriptomes[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 663-672.

References 30

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Effects of gga-miR-155 on MDCC-MSB1 Cell Transcriptomes

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