基于转录组测序研究绿光影响鹅胚心脏早期发育的候选基因

doi:10.11843/j.issn.0366-6964.2024.05.016

Abstract

Abstract: The aim of this study was to identify candidate genes for heart development in goose embryos by analyzing the gene expression differences in embryonic hearts tissue under green light stimulation. The 512 goose eggs were randomly divided equally into green light and dark incubators, with 4 layers and 64 eggs per layer placed in each incubator. During the entire incubation period, the green light incubator provided intermittent light regimen of 15 minutes on and 15 minutes off. On the 13, 16, 20, 24, 28, and 30 day of incubation, 8 eggs were randomly selected from each group, the embryo weight was measured, and the embryonic heart tissue was separated and weighed. The embryonic heart tissues were collected at day13 of incubation, and transcriptome sequencing (RNA-Seq) were performed. Differentially expressed genes (DEGs) were screened, and subjected to functional enrichment analysis to identify candidate genes related to green light promoting heart development. And the reliability of the sequencing results was validated using fluorescence quantitative PCR (qRT-PCR). The green light significantly increased the proportion of embryo weight at 16 and 30 embryonic ages (embryo weight/hatched egg weight×100) (P<0.05), and the heart index (heart weight/embryo weight×100) at 13 and 16 embryonic ages in the green light group were significantly higher than individuals in the dark group (P<0.05). A total of 1 643 DEGs were screened from the green light group and the dark group, 7 DEGs were randomly selected for RT-qPCR validation, and the expression trend was consistent with the sequencing results. Enrichment analysis showed that DEGs were mainly enriched in the PI3K-Akt signaling pathway, AMPK signaling pathway, and other pathways. Four candidate genes: GATA4, GATA5, Smad4 and GHR were ultimately screened out. Their expression in heart tissues at different embryonic ages were analyzed to further validated their regulatory role in heart development. Through the analysis of transcriptomic data of heart tissue, we identified GATA4, GATA5, Smad4 and GHR as candidate genes for goose embryonic heart development, which provide clues to the molecular regulatory mechanism of green light on embryonic heart development, and provide a theoretical basis for the application of green light in goose egg hatching.

Key words: goose, green light, incubation, heart, transcriptome, GATA4

CLC Number:

S835.2

CHEN Zhe, QU Xiaolu, GUO Binbin, SUN Xuefeng, YAN Leyan. Study on Candidate Genes for Green Light Affecting Early Development of Goose Embryo Heart Based on Transcriptome Sequencing[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 1978-1988.

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Study on Candidate Genes for Green Light Affecting Early Development of Goose Embryo Heart Based on Transcriptome Sequencing

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