CRISPR/Cas9技术在动物非编码RNA功能研究中的应用

doi:10.11843/j.issn.0366-6964.2020.04.001

Abstract

Abstract: CRISPR/Cas9 system is an immune mechanism that widely exists in bacteria and archaea. In recent years, it has been used as an efficient gene editing tool to investigate the function of coding or non-coding RNA. Non-coding RNA is a type of RNA that is not translated into proteins, and it plays an important role in biological processes such as animal growth, development and disease immunity through a variety of regulatory pathways. CRISPR/Cas9 technology can be used for targeting nucleotide sequence to get stable knockout mice or cell lines. When it is used in the research of non-coding RNA, it may interfere the expression of neighbor genes or host genes. Even though it has some shortages, this technology provides different ways for exploring the functional mechanism of non-coding RNA. In this review, we briefly summarize the history and functional principle of CRISPR/Cas system, and focus on its application in the functional research of miRNA, lncRNA and circRNA in animals in order to provide references for related research.

Key words: CRISPR/Cas9 technology, non-coding RNA, miRNA, lncRNA, circRNA, animal

CLC Number:

XU Xiaoli, CAO Jiaxue. Application of CRISPR/Cas9 Technology in the Study of Animal Non-coding RNA Function[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(4): 649-659.

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Application of CRISPR/Cas9 Technology in the Study of Animal Non-coding RNA Function

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