饲喂青贮黄梁木代替青贮玉米川中黑山羊肝转录组的表达分析

doi:10.11843/j.issn.0366-6964.2024.01.022

Abstract

Abstract: The purpose of this study was to analyze the differentially expressed mRNA, lncRNA and its target genes in liver transcripome sequencing of Chuanzhong Black Goats fed silage Neolamarckia Cadamba instead of 50% silage corn, and to find out the key genes affecting liver metabolism of Chuanzhong Black Goats fed silage Neolamarckia Cadamba, so as to explore the feeding effect of silage Neolamarckia Cadamba. A total of 6 healthy male Chuanzhong Black Goats [5 months old, average body weight (21.07±4.05) kg] were selected and randomly divided into two groups: experimental group was fed silage Neolamarckia Cadamba replaced 50% silage corn, and control group was fed silage corn. Total mixed diet (TMR) was used for feeding during the experiment. On the last day of the experiment period, goats were slaughtered and their livers were taken. RNA from the liver tissues was extracted to construct a library. High-throughput sequencing technology was used for transcriptome sequencing. Differential mRNA and lncRNA were screened at a threshold of P<0.05, and biological function enrichment analysis was conducted to construct a targeted relationship network. Finally, 3 genes were randomly selected from differentially expressed lncRNA and mRNA separately, and verified by RT-qPCR. The results showed that a total of 1 696 mRNA were differentially expressed, of which 943 were up-regulated and 753 were down-regulated. A total of 33 lncRNA were differentially expressed, of which 22 were up-regulated and 11 down-regulated. Combined with the enrichment analysis of target genes of differentially expressed lncRNA and intersection genes of differentially expressed mRNA, it was found that NDUFA10, NDUFB11 and NDUFS5 were involved in signal pathways related to liver function, such as polysaccharide metabolism, glucan catabolism and cellulose catabolism. Combined with lncRNA target gene prediction, it was found that GSTA3 and GSTA4 genes were involved in signal pathways related to liver function, such as drug metabolism-cytochrome P450 and cytochrome P450 metabolism of heterologous substances. In conclusion, NDUFA10, NDUFB11, NDUFS5, GSTA3 and GSTA4 may affect the liver function from the aspects of nutrient metabolism and toxin metabolism in the liver tissue of Chuanzhong Black Goats fed with silage Neolamarckia Cadamba.

Key words: silage Neolamarckia Cadamba, Chuanzhong Black Goat, liver, lncRNA, mRNA

CLC Number:

S813

ZHANG De'an, YANG Ruozhu, LIU Jie, LIU Dewu, DENG Ming, LIU Guangbin, SUN Baoli, GUO Yongqing, LI Yaokun. Expression Analysis of Transcriptome in the Liver of Chuanzhong Black Goats Fed with Silage Neolamarckia Cadamba Substitute for Silage Corn[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 232-244.

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Expression Analysis of Transcriptome in the Liver of Chuanzhong Black Goats Fed with Silage Neolamarckia Cadamba Substitute for Silage Corn

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