不同FecB基因型和不同直径绵羊卵泡中BMP/SMAD通路活性及蛋白表达差异

doi:10.11843/j.issn.0366-6964.2024.09.020

Abstract

Abstract:

This study aimed to investigate the effects of different FecB genotypes on the BMP/SMAD signaling pathway activity and protein expression in sheep follicles, and explored the differences in BMP/SMAD signaling pathway activity and protein expression between mature large follicles and small follicles. Firstly, TaqMan method was used to screen ewes with different genotypes of FecB. Secondly, after estrus synchronization, mature follicles in follicular phase and small follicles in luteal phase were sampled, and the pathway activity and the protein expression of BMP/SMAD pathway were determined by Western blot. The result showed that, for the small follicle group, the expression of bone morphogenetic protein receptor type 1B (BMPR1B) in FecB mutant follicles was significantly higher than that in wild-type follicles (P<0.05), but the expression of SMAD family member 4 (SMAD4) and the phosphorylation levels of SMAD1/5/9 were significantly lower than those in wild-type follicles (P<0.05). For the mature large follicle group, the expression of FKBP prolyl isomerase 1A (FKBP1A) and SMAD4 in FecB mutant follicles was significantly lower than that in wild-type follicles (P<0.05), and there was no significant difference in the protein expression of type Ⅰ receptor (BMPR1B) and type Ⅱ receptor (BMPR2) and the phosphorylation levels of SMAD1/5/9 between the two genotypes. On the other hand, when comparing FecB mutant small follicles with mature large follicles, the protein expression levels of BMPR1B and SMAD4 and the phosphorylation level of SMAD1/5/9 in mature large follicles were significantly higher than those in small follicles (P<0.05). The results indicated that due to the decrease in the expression of SMAD4, the FecB mutant large and small follicles have relatively fewer SMAD4-SMAD1/5/9 protein complexes bound to the genomic target regions, which means a reduction in pathway activity. Moreover, due to the lower phosphorylation level of SMAD1/5/9 in small follicles, its pathway activity was lower. Additionally, after the development and maturation of the mutant sheep follicles, the activity of the BMP/SMAD pathway was significantly enhanced.

Key words: sheep, FecB mutation, follicle, BMP/SMAD pathway, SMAD4

CLC Number:

S826.3

Yiming GONG, Yixuan JIA, Jiajun LI, Xiangyu WANG, Xiaoyun HE, Mingxing CHU, Ran DI. BMP/SMAD Pathway Activity and Protein Expression Profiles in Ovarian Follicles with Different Diameters in Diverse FecB Genotyped Ewes[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(9): 3957-3967.

Figures/Tables 13

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分组 Category	基因型 Genotype	卵巢个数 Amount of ovary	卵泡直径(平均值±标准差) Diameter of ovarian follicle (Average ±Standard deviation)	每只羊采样卵泡数量 Amount of ovarian follicle collected from each sheep
大卵泡组	FecB野生型	12	6.00±0.74^a	直径>5 mm的1个
Large follicle group	FecB突变型	12	4.40±0.42^b	直径>4 mm的2个
小卵泡组	FecB野生型	12	2.42±0.19	直径 < 3 mm的5个以上
Small follicle group	FecB突变型	12	2.52±0.18	直径 < 3 mm的5个以上

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BMP/SMAD Pathway Activity and Protein Expression Profiles in Ovarian Follicles with Different Diameters in Diverse FecB Genotyped Ewes

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