肉羊体外胚胎生产技术规模化应用中品种的影响

doi:10.11843/j.issn.0366-6964.2024.06.017

Abstract

Abstract:

The aim of this study was to explore the impact of breed factors on the efficiency of commercial rapid propagation of OPU-IVEP. This study used healthy 8-month-old to 3-year-old black headed Suffolk, black headed Dorper, and Texel meat breeding ewes identified by breed identification as donors. OPU technology was used to obtain oocytes at Inner Mongolia Saino Sheep Technology Co., Ltd. for in vitro embryo factory production or in vivo embryos obtained through laparoscopic insemination and surgical flushing. Embryo transfer was performed, and relevant data of oocyte recovery, embryo development, and transfer pregnancy were recorded for statistical analysis. The differences in oocyte recovery efficiency, oocyte in vitro fertilization and embryo development ability, embryo transfer pregnancy and lambing efficiency among different breeds in large-scale in vitro embryo production was compared; At the same time, the application effects of in vivo and in vitro embryo production technologies of different breeds were also compared. The results showed that: 1) there was a significant difference in oocyte recovery rates among the 3 breeds (P < 0.01), with the highest recovery efficiency observed in the black headed Dorper; 2) There was no significant difference in the in vitro fertilization and cleavage rates among the 3 breeds (P > 0.05), but the blastocyst development rate of Texel sheep was significantly higher than the other 2 breeds (P < 0.01); 3) There was no significant difference (P > 0.05) in the conception rate and average number of lambs produced by embryo transfer between different breeds, whether in vivo or in vitro, and there was no significant difference (P > 0.05) in the average conception rate and lambing rate of in vivo and in vitro embryo transfer in different breeds. In summary, in the process of OPU-IVEP industrial and large-scale application in sheep in vitro embryo production, there are differences in the efficiency among different breeds, such as follicle development, number of retrieved oocytes, and blastocyst development rate. Texel sheep, which has the lowest average number of follicles and average number of retrieved oocytes, has the highest blastocyst development rate; The conception and lambing situation after transplantation, which directly affects the efficiency of rapid reproduction, did not show significant differences among different varieties, and there was no significant difference in the number of offspring obtained from single production of in vivo and in vitro embryos. However, the donor utilization rate of OPU-IVEP is higher than that of MOET.

Key words: breed, meat sheep, in vitro embryo production, embryo transfer, scaling, application

CLC Number:

Ying CHEN, Dayong CHEN, Riga WU, Chunjuan QIU, Lihong FAN, Meirong BAO, Yuan YUE, Hongyan LIANG, Jiaxin ZHANG, Jianhui TIAN, Lei AN, Liqin WANG. Influence of Meat Sheep Varieties on the Scale Application of in vitro Embryo Production Technology[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(6): 2451-2459.

Figures/Tables 4

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References 41

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品种 Breed	实施OPU总数/次 Total number of OPU implemented	卵泡数/个 No. of follicles	平均卵泡数/(个·次^-1) Average number of follicles	回收卵数/枚 No. of retrieved oocytes	平均回收卵数/(枚·次^-1) Average number of retrieved oocytes
黑头萨福克BS	977	16 368	17.12±0.65^ABa	16 835	17.44±0.54^A
黑头杜泊BD	686	12 723	18.87±0.75^Aa	14 257	21.19±0.77^B
德克赛尔DK	112	1 570	13.97±0.14^Bb	1 490	13.20±0.33^C
合计Total	1 775	30 661	17.49±0.52	32 582	18.54±0.66

品种 Breed	卵母细胞数/枚 No. of oocytes	卵裂胚胎数/枚 No. of cleavage embryos	卵裂率/% Cleavage rate	培养胚胎数/枚 No. of cultured embryos	囊胚数/枚 No. of blastocysts	囊胚率/% Blastocyst rate
黑头萨福克BS	22 043	16 596	76.77±3.15	9 727	5 478	57.06±1.31^A
黑头杜泊BD	15 947	11 097	72.94±2.76	8 464	4 359	53.54±2.55^A
德克赛尔DK	2 113	1 625	71.98±2.00	1 339	870	69.09±4.25^B
合计Total	40 103	29 318	73.11	19 530	10 707	54.82

品种 Breed	供体数/只次 No. of donors	移植胚胎数/只 No. of transferred embryos	移植受体数/只 No. of transplant recipients	怀孕母羊数/只 No. of pregnant ewes	移植受胎率/% Transplantation conception rate	产羔数/只 No. of lambs produced	平均产羔数/只 Average number of lambs per donor
黑头萨福克BS	1 532	9 264	5 230	3 790	75.20±1.33	5 058	3.33±0.19
黑头杜泊BD	605	2 525	1 813	1 244	70.39±2.80	1 903	3.15±0.36
德克赛尔DK	513	2 616	1 656	1 144	72.69±3.56	1 731	3.36±0.33
合计Total	2 650	1 4405	8 699	6 178	71.02	8 692	3.28

品种 Breed	供体数/只次 No. of donors	可用胚胎数/枚 No. of available embryos	平均胚胎数 Average number of embryos	移植受体数/只 No. of transplant recipient	怀孕母羊数/只 No. of pregnant ewes	移植受胎率/% Transplantation conception rate	产羔数/只 No. of lambs produced	平均产羔数/只 Average number of lambs per donor
黑头萨福克BS	515	1 860	4.17±1.86	1 289	997	71.64±5.66	1 116	2.52±1.11
黑头杜泊BD	4 825	25 946	5.71±0.44	16 683	11 890	71.59±2.33	15 573	3.43±0.27
德克赛尔DK	139	679	4.91±1.40	573	371	64.62±4.77	418	3.01±0.80
合计Total	5 479	28 485	5.20	18 545	13 258	71.49	17 107	3.12

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Influence of Meat Sheep Varieties on the Scale Application of in vitro Embryo Production Technology

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