Gα12蛋白抑制口蹄疫病毒在PK-15细胞上复制

doi:10.11843/j.issn.0366-6964.2020.03.015

Abstract

Abstract: The guanine nucleotide binding protein Gα12 is involved in the regulation of various cellular responses, but there are few studies on foot-and-mouth disease virus (FMDV). In this study, we had used FMDV to infect PK-15 cells, and found that the mRNA and protein levels of Gα12 were up-regulated, indicating that Gα12 may have some relationships with FMDV replication. To investigate the effect of Gα12 on the replication of FMDV, quantitative real-time PCR, Western blot and TCID₅₀ were used to detect the effect of overexpression of Gα12 and knockdown of Gα12 on FMDV replication. The results showed that the mRNA level, protein level and virus titer of FMDV were decreased after overexpression of Gα12. However, when knockdown Gα12, the replication of FMDV showed the opposite effect, indicating that Gα12 inhibited the replication of FMDV. This study found for the first time that Gα12 has the effect of inhibiting the replication of FMDV, and provides a new idea and theoretical basis for the study of anti-FMDV, which is of great significance for FMVD-related research.

Key words: Gα12, foot-and-mouth disease virus, replication

CLC Number:

S852.659.6

WANG Zhifang, ZHANG Wei, YANG Fan, WEI Ting, ZHENG Haixue. Gα12 Suppresses the Replication of Foot-and-mouth Disease Virus in PK-15 Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(3): 556-564.

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Gα12 Suppresses the Replication of Foot-and-mouth Disease Virus in PK-15 Cells

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