嵌合口蹄疫病毒抗原表位的重组塞内卡病毒A的构建及其鉴定

doi:10.11843/j.issn.0366-6964.2020.03.016

Abstract

Abstract: It is difficult to distinguish the clinical symptoms caused by Seneca virus A (SVA) and foot-and-mouth disease virus (FMDV), and the study of inserting foreign genes of FMDV epitope with SVA as avector has not been reported at present. This paper aims to construct a recombinant SVA strain with chimeric FMDV epitopes and identify it. The genes encoding B cell epitopes of type O FMDV and albumin signal peptide (Human albumin signal peptide, HAS) were inserted into the SVA genome by gene cloning technique. The recombinant plasmid was successfully constructed and the recombinant virus was rescued after transfection. The results of RT-PCR and gene sequencing showed that the target gene was inserted correctly, and the effective expression of chimeric antigen protein was identified by Western blot and cellular indirect immunofluorescence. Genetic stability analysis showed that the recombinant virus still had stable B cell epitopes during the passage of 25 generations. The results of plaque phenotype and one-step growth curve of the virus showed that the proliferation characteristics of the recombinant virus were similar to those of the parent strain. In this study, the recombinant SVA virus which can express FMDV epitopes was successfully constructed and rescued, which provides a theoretical and experimental basis for the research of chimeric virus and vaccine based on SVA.

Key words: Seneca virus A, foot-and-mouth disease virus, antigen epitope, chimeric virus

CLC Number:

S852.659.6

SONG Gaoyuan, YANG Fan, HAO Rongzeng, LI Yu, ZHENG Haixue. Construction and Identification of Recombinant Seneca Virus A of Chimeric Foot-and-mouth Disease Virus Antigenic Epitope[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(3): 565-573.

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Construction and Identification of Recombinant Seneca Virus A of Chimeric Foot-and-mouth Disease Virus Antigenic Epitope

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