猪GRK2蛋白抗口蹄疫病毒作用分析

doi:10.11843/j.issn.0366-6964.2023.10.031

Abstract

Abstract: To investigate the effect of G protein-coupled receptor kinase 2 (GRK2) on the replication of foot-and-mouth disease virus (FMDV), the porcine cellular RNA from PK-15 cells were extracted and the complete CDS region of GRK2 was obtained by Polymerase Chain Reaction (PCR) methods. Subsequently, the eukaryotic expressing plasmid was constructed. The Western blot and indirect immunofluorescence experiments showed that GRK2 was expressed in the transfected cells and located in the cytoplasm. The infection assay suggested that the transcription level and protein level of GRK2 firstly increased and then decreased in FMDV-infected PK-15 cells. Overexpression assay indicated that GRK2 significantly inhibited FMDV replication, and siRNA assay showed that downregulation of GRK2 promoted FMDV replication, revealing the antiviral function of GRK2 against FMDV. Further studies showed that FMDV 3C^pro protein interacted with GRK2 and degraded GRK2 to promote FMDV replication. In conclusion, this study laid a foundation for analysis of the antiviral mechanism of host protein GRK2 against FMDV.

Key words: foot-and-mouth disease virus, G protein-coupled receptor kinase 2, antiviral effect

CLC Number:

S852.659.6

CHEN Wenzhe, ZHANG Xiangle, GU Fengxing, ZHAO Zhenxiang, LI Kangli, XUE Zhaoning, ZHENG Haixue, ZHANG Xiaoli, ZHU Zixiang. Evaluation of the Antiviral Effect of Porcine GRK2 against Foot-and-mouth Disease Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4350-4361.

References 30

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Evaluation of the Antiviral Effect of Porcine GRK2 against Foot-and-mouth Disease Virus

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