生物矿化的口蹄疫病毒样颗粒疫苗的免疫原性评价

doi:10.11843/j.issn.0366-6964.2023.04.023

Abstract

Abstract: Biomineralization technology can enhance the heat resistance of foot-and-mouth disease (FMD) virus-like particles (VLPs), but there is no experimental data to support the feasibility of mineralized FMD VLPs for clinical use. The aim of this study was to evaluate the immunogenicity of the mineralized FMD VLPs vaccine in cattle and pigs. The pigs were immunized with VLPs and mineralized VLPs, and the specific antibody titers in the serum of the immunized pigs were detected by liquid-phase blocking ELISA (LPBE). The specific IFN-γ secreted by peripheral blood lymphocytes (PBLs) after stimulation in vitro was detected by enzyme-linked immunospot assay (ELISpot). Cattle were immunized with mineralized type A VLPs, mineralized type O VLPs, and mineralized type A and type O bivalent VLPs, respectively. The serum specific antibody, neutralizing antibody, IL-4 and IFN-γ contents were detected by LPBE, neutralization test and sandwich ELISA. The results showed that there was no significant difference in serum antibody level between VLPs and mineralized VLPs immunized pigs.Compared with VLPs, the level of IFN-γ secreted by PBLs of mineralized VLPs immunized pigs was higher than that of VLPs immunized pigs (P<0.05). The serum of cattle immunized with the three vaccines contained more IL-4 and IFN-γ (P<0.05), and there were no significant differences in the titers of serum specific antibodies and neutralizing antibodies between the mineralized bivalent vaccine and the corresponding mineralized monovalent vaccine. In conclusion, mineralized VLPs have good immunogenicity for both cattle and pigs, mineralized VLPs of different serotypes can be used in combination, and mineralized shells can enhance the ability of VLPs to induce cellular immune response.

Key words: foot-and-mouth disease virus, virus-like particles vaccine, biomineralization, animal immunization

CLC Number:

S852.4

LI Shuo, ZHANG Yun, BAI Manyuan, ZHAO Ruichong, SONG Hetao, MU Suyu, TENG Zhidong, DONG Hu, MA E'ning, SUN Shiqi, GUO Huichen, YIN Shuanghui. Immunogenicity Evaluation of Biomineralized Foot-and-mouth Disease Virus-like Particles Vaccine[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1598-1607.

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Immunogenicity Evaluation of Biomineralized Foot-and-mouth Disease Virus-like Particles Vaccine

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