黄芩苷通过促进Ⅰ型干扰素表达抑制鸡传染性支气管炎病毒复制

doi:10.11843/j.issn.0366-6964.2023.11.037

Abstract

Abstract: This study aimed to investigate the inhibitory effect of baicalin on IBV and its underlying mechanism. Firstly, baicalin (125 μg·mL^-1)was added into cells (H1299 and Vero) before or after IBV infection to analyze the inhibitory effect of baicalin on IBV replication, and then baicalin was diluted into different concentrations to observe the inhibitory effect of different concentrations of baicalin on IBV replication. Furthermore, the experiment was divided into normal group, IBV group, pre-treatment group and post-treatment group, qRT-PCR and Western blot were used to detect mRNA and protein expressions of IFN pathway related signaling molecules in each group. At the same time, the sensitivity of IBVs to IFN treatment was analyzed after treatment with recombinant human IFN-α and the nuclear translocation of p-STAT1 was detected by indirect immunofluorescence in IBV group and dosed groups. Finally, the effect of STAT1 silencing on IBV replication was analyzed by gene silencing assay. The results showed that baicalin had an inhibitory effect on IBV replication in a dose-dependent manner. Baicalin treatment up-regulated the expressions of IFN pathway signaling molecules P-IRF3, IFN-β, p-STAT1 (P<0.05), down-regulated the expression of JAK-STAT signaling pathway negative regulator SOCS3 and SOCS3 stimulator IL-6 (P<0.05). Simultaneously, baicalin could increase IFN-mediated translocation of p-STAT1 and increase the sensitivity of IBV to IFN treatment. Further experiments revealed that silencing STAT1 decreased the inhibition effect of baicalin on IBV replication. The results showed that baicalin can inhibit IBV replication and reduce the pathological effect of viral infection on host cells by inducing type Ⅰ IFN production.

Key words: baicalin, infectious bronchitis virus(IBV), replication, interferon

CLC Number:

JIA Yiquan, YU Mengting, LIU Weirong, FANG Shouguo, ZHANG Songbai. Baicalin Inhibits Infectious Bronchitis Virus Replication via Promoting the Expression of Type Ⅰ Interferon[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4839-4850.

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Baicalin Inhibits Infectious Bronchitis Virus Replication via Promoting the Expression of Type Ⅰ Interferon

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