白细胞介素-10对口蹄疫病毒感染小鼠T细胞增殖及其表达TNF-α、IFN-γ和IL-2的影响

doi:10.11843/j.issn.0366-6964.2023.02.026

Abstract

Abstract: The increase of interleukin-10 (IL-10) is one of the hallmarks during foot-and-mouth disease virus (FMDV) infection. The aim of this study was to investigate the effects of IL-10 on the proliferation and the expressions of function-related cytokines of T cells in peripheral blood of mice infected with FMDV. The proliferation and the expressions of effector function-related cytokines (TNF-α, IFN-γ and IL-2) of T cells in peripheral blood of mice were detected by CCK-8 and flow cytometry, respectively. The results showed that the proliferations of T cells in peripheral blood of FMDV-infected mice at 12, 24, 36 and 48 hpi were significantly decreased compared with that of mock-mice (P<0.05 or P<0.01); and the expressions of TNF-α and IL-2 of CD4⁺ T cells in peripheral blood of infected mice were significantly decreased compared with that of mock-mice (all P<0.01); and the expressions of TNF-α, IFN-γ and IL-2 of CD8⁺ T cells in peripheral blood of infected mice were also significantly decreased compared with that of mock-mice (P<0.01 or P<0.000 1). However, blocking IL-10/IL-10R signaling in vivo or knocking out IL-10 contributed to restore the proliferation of T cells in peripheral blood following FMDV infection (P<0.05 or P<0.01), but did not affect the expressions of TNF-α, IFN-γ and IL-2 of CD4⁺ and CD8⁺ T cells. The study for the first time revealed that the proliferation and the expressions of TNF-α, IFN-γ and IL-2 of T cells were inhibited after FMDV infection, which contributed to evade immune responses. Although blocking IL-10/IL-10R signaling in vivo or knocking out IL-10 did not affect the expressions of TNF-α, IFN-γ and IL-2 of CD4⁺ and CD8⁺ T cells, it contributed to restore the proliferation of T cells in peripheral blood of FMDV-infected mice, providing new perspectives and new strategies for the design of new products for FMD prevention and control.

Key words: foot-and-mouth disease virus, interleukin-10, T cells, proliferation, cytokine

CLC Number:

S852.659.6

GUO Zijing, CHEN Fei, ZHANG Zhixiong, BAI Ling, ZHANG Zhidong, LI Yanmin. Effects of Interleukin-10 on T Cell Proliferation and Its Expression of TNF-α, IFN-γ and IL-2 in Mice Infected with Foot-and-Mouth Disease Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 694-705.

References 29

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Effects of Interleukin-10 on T Cell Proliferation and Its Expression of TNF-α, IFN-γ and IL-2 in Mice Infected with Foot-and-Mouth Disease Virus

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