Acta Veterinaria et Zootechnica Sinica ›› 2022, Vol. 53 ›› Issue (7): 2252-2259.doi: 10.11843/j.issn.0366-6964.2022.07.021

• PREVENTIVE VETERINARY MEDICINE • Previous Articles     Next Articles

β-estradiol and Progesterone Promote Replication of African Swine Fever Virus in vitro

YUAN Xingguo1,2, SHEN Chaochao2, ZHAO Dengshuai1,2, YANG Bo2, CUI Huimei1,2, HAO Yu2, YANG Jinke2, CHEN Xuehui2, ZHANG Ting1,2*, ZHANG Keshan1*, LIU Xia2, ZHENG Haixue2   

  1. 1. College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070;
    2. State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine of Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou 730000, China
  • Received:2021-10-27 Online:2022-07-23 Published:2022-07-23

Abstract: This study aimed to investigate the effect of estradiol (E2) and progesterone (P4) on the replication of African swine fever virus (ASFV) in vitro. Non-pregnant swine serum (NPSS) and pregnant swine serum (PSS) were collected and treated. Porcine alveolar macrophages (PAMs) and Bone marrow-derived macrophages (BMDM) were cultivated with Fetal bovine serum (FBS), NPSS and PSS respectively and infected with ASFV. The difference of ASFV replication were detected by absolute quantitative PCR and Western blot. Using E2 and P4 ELISA kits to detect the contents of E2 and P4 in each serum. ASFV was infected with E2 and P4 alone or in combination, and the replication differences of ASFV were detected by absolute quantitative PCR and Western blot. NPSS and PSS treated BMDM for 24 hours, the transcriptional difference of beta-interferon and downstream antiviral factors was detected by relative quantification. The results showed that ASFV replication in PSS group was more effective than NPSS group. The contents of E2 and P4 in PSS were higher than NPSS, but the contents of E2 and P4 in FBS were low levels, and ASFV replication is more effective after adding E2 and P4 alone or mixed in FBS group. And PSS treatment of BMDM can inhibit the transcription of beta-interferon and downstream antiviral factors. High levels of E2 and P4 in PSS promote ASFV replication in vitro. This study provides a scientific basis for explaining the African swine fever (ASF) occurs preferentially in sows and symptoms are more serious in pregnant sows.

Key words: African swine fever virus, estradiol, progesterone, in, vitro, promote replication

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