表达水泡性口炎病毒双血清型G蛋白重组腺病毒的构建及对小鼠的免疫原性分析

doi:10.11843/j.issn.0366-6964.2015.12.014

Abstract

Abstract:

The purpose of this study was to construct the recombinant adenovirus expressing two serotypes of vesicular stomatitis virus(VSV) G proteins with human adenovirus expression vector.The gene VSV-IN-G-NJ-G was amplified by fusion-PCR and cloned into the adenovirus shuttle vector pacAd-CMV K-NpA.The recombinant shuttle plasmid and adenovirus backbone plasmid linearized by digestion with PacⅠ，and then were transfacted into AAV-293 cells to obtain recombinant adenovirus rAd-VSV-IN-G-NJ-G.The recombinant adenovirus was stable in AAV-293 cells after serial passage to 20 generations.By indirect immunofluorescence and western blot detection，G proteins were expressed in recombinant adenovirus.The mice were inoculated with recombinant adenovirus for evaluating the humoral immune responses by indirect ELISA and neutralization experiment.The results showed that the recombinant adenovirus could induce VSV specific antibodies and the neutralizing antibody level of inoculated group was 1：32.The result of cellular immune showed that the recombinant adenovirus can cause a strong lymphocyte proliferation of the inoculated mice.These results suggested that the recombinant adenovirus can cause a certain degree of humoral and cellular immune responses in inoculated mice.The research laid foundations for further studies of VSV gene-engineering vaccines.

CLC Number:

S852.659.5

XUE Xiao-juan，JIN Hong-yan，ZHAO Ling-na，SUI Xiu-kun，Zheney Makay，LI Gang. Construction and Immunogenicity of Recombinant Adenovirus Expressing Two Serotypes of Vesicular Stomatitis Virus G Proteins in Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(12): 2227-2234.

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Construction and Immunogenicity of Recombinant Adenovirus Expressing Two Serotypes of Vesicular Stomatitis Virus G Proteins in Mice

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