细胞自噬对牛病毒性腹泻病毒复制的影响

doi:10.11843/j.issn.0366-6964.2015.07.017

Abstract

Abstract:

The objective of this study was to investigate the role of cellular autophagy in bovine viral diarrhea virus (BVDV) infection.We used Oregon C24V strain to determine whether BVDV infection induced autophagy by using western blot，indirect immunofluorescent assay and transmission electron microscopy.To analyze the effect of autophagy on the replication of BVDV in MDBK cell，wortmannin and small interfering RNAs targeting Beclin-1 were used to inhibit autophagy.The results showed that BVDV infection results in LC3-Ⅰ/Ⅱ conversion，p62 degradation，an increased accumulation of punctate GFP-LC3-expressing cells，and a higher number of autophagosome-like double-membrane vesicles in the cytoplasm of host cells.Inhibition of autophagy or interfering expression of Beclin-1 led to a significant reduction in BVDV titers and protein expression.These results demonstrate that BVDV infection induces autophagy which in turn enhances BVDV replication.

CLC Number:

S852.659.6

GONG Xiao-wei，ZHENG Fu-ying，CHEN Qi-wei，LIU Yong-sheng，LI Zhao-cai，ZHOU Ji-zhang，CAI Xue-peng，YIN Hong. Impact of Cellular Autophagy on Bovine Viral Diarrhea Virus Replication[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1201-1207.

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Impact of Cellular Autophagy on Bovine Viral Diarrhea Virus Replication

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