不同毒力和剂量的新城疫病毒感染鸡胚后固有免疫相关分子mRNA转录水平的变化

doi:10.11843/j.issn.0366-6964.2016.12.017

Abstract

Abstract:

Newcastle disease virus (NDV) is the pathogen of Newcastle disease (ND), which causes serious economic losses to poultry industry. For a better understanding of innate immune response in the pathogenesis of NDVs, SPF chicken embryos were infected with a virulent NDV strain and an avirulent vaccine strain-LaSota, respectively, with different infection doses set for both viruses. At the early stage of viral infection, the dynamics of viral proliferation and the transcriptional level of innate immune-related molecules were tested with fluorescence quantitative PCR assays. The results showed that both the virulent and avirulent NDV strains could activate the innate immune signaling pathways, and resulted in the transcriptional changes of cellular pattern recognition receptors, interferons, interleukins and antiviral proteins. NDV infection of high doses in comparison with low doses resulted in quicker viral proliferation and earlier peaks of innate immune-related gene expression. The virulent NDV strain induced higher levels of interferons and inflammatory cytokines in chicken embryos, which was consistent with the severe inflammation and pathological damages of both chicken embryos and chickens caused by virulent viral infection. In conclusion, this study found that the response patterns of chicken innate immune signaling pathways varied due to NDV virulence and infection dose, and the genotype VII NDV strain could induce strong cellular inflammatory responses, which may contribute to its high pathogenicity to chickens.

CLC Number:

S852.659.5

GAO Dan-dan, HUANG Yan-yan, XU Chuan-tian, YANG Shao-hua, HUANG Qing-hua, ZHANG Lin，ZHANG Xiu-mei, WU Jia-qiang, LI Jian-liang, CUI Yan-shun. Transcriptional Responses of Innate Immunity Related mRNAs after Infection of Embryonated Chicken Eggs with Newcastle Disease Viruses of Variant Virulence and Doses[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(12): 2469-2475.

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Transcriptional Responses of Innate Immunity Related mRNAs after Infection of Embryonated Chicken Eggs with Newcastle Disease Viruses of Variant Virulence and Doses

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