小反刍兽疫病毒化学合成表位多肽对小鼠的免疫效果研究

doi:10.11843/j.issn.0366-6964.2015.03.013

Abstract

Abstract:

Epitope polypeptides of peste des petits ruminants virus (PPRV) were synthesized and its immunogenicity was examined in mice.Four epitope polypeptides against F and H gene of PPRV were selected by the epitope prediction software and acquired by chemical synthesis，then were administered subcutaneously at dose of 100 μg to BALB/c mice，and the epitope antigenicity was analyzed by immunoassays of ELISA，MTT，the killing activity of NK cell and T lymphocyte subsets detection.As a result，H242-255，F528-541 and H349-362 had better humoral immune function among the four epitope polypeptides，while H242-255 and H392-405 had better cellular immune function.Thus，peptide of H242-255 can satisfy the two immunologic functions，and can be used as a candidate epitope vaccine component.The study had great significance by screening efficient epitopes for the development of PPRV epitope vaccines.

CLC Number:

S852.659.5

LIU Wen-hua，WANG Zhi-liang，WU Xiao-dong，SUN Cheng-you. Research of the Immune Efficacy of Chemical Synthetic Epitope Polypeptide of Peste des Petits Ruminants Virus on Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(3): 438-444.

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Research of the Immune Efficacy of Chemical Synthetic Epitope Polypeptide of Peste des Petits Ruminants Virus on Mice

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