野生水禽源ClassⅠ新城疫病毒对SPF鸡的致病性研究

doi:10.11843/j.issn.0366-6964.2015.09.016

Abstract

Abstract:

A lentogenic Newcastle disease virus (NDV)，SD22/13，was isolated from heron.The biology and pathogenicity were characterized in order to understand the evolution of NDV better.F gene of SD22/13 was sequenced and compared with that of reference strains.The antigenicity of the virus was compared with that of LaSota and other genotype Ⅶ virulent NDV strains via cross hemagglutination inhibition (HI).The pathogenicity of SD22/13 to SPF chicken was also conducted.The results indicated that SD22/13 was classified as a class Ⅰ genotype 3 NDV based on a partial sequence of the F gene.The antigenicity correlation between SD22/13 and LaSota was 56.1%，and the correlation of which with genotype Ⅶ NDV strains was less than 50%.Experiments on animals demonstrate that the isolate SD22/13 can replicate well in SPF chicken and induce a high immune antibody and had slight pathogenic to immune organ and kidney.Chickens inoculated with SD22/13 were challenged with genotype Ⅶ virulence NDV strain on 30dpi，and there had no death in chickens infected with SD22/13.The results demonstrated that SD22/13 stain had significant differences with genotypeⅦ NDV in biological characterization and pathogenic to chickens.Although the antigenic correlation was low between SD22/13 and genotype Ⅶ NDV，class I viruses can provide 100% protection rate，but it cannot block virus shedding upon challenge with genotypeⅦ virulent NDV.

CLC Number:

S852.659.5

YANG Shao-hua，XU Chuan-tian，HUANG Yan-yan，ZHANG Lin，HUANG Qing-hua，HU Bei-xia，ZHU Man-ling，ZHANG Xiu-mei. Research on Pathogenicity of SPF Chicken Infected with a Class Ⅰ NDV Isolated from Waterfowl[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1606-1612.

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Research on Pathogenicity of SPF Chicken Infected with a Class Ⅰ NDV Isolated from Waterfowl

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