牛狂犬病病毒的鉴定及N基因序列分析

Abstract

Abstract:

To diagnose suspected cattle rabies case and analyze molecular characteristics of the pathogen, brain tissue materials of cattle with clinical symptom from a farm in China were used. Rabies virus (RV) in brain tissue was confirmed by Mouse Inoculation Test (MIT), Hematoxylin and Eosin Staining (HE), Fluorescent antibody test (FAT) and specific nucleoprotein gene (N) RT-PCR amplification. Molecular characterization of RV strain was performed using DNAStar, Mega4.0 softwares based on N gene sequence. Results showed that this cattle disease case was caused by RV, phylogenetic tree analysis demonstrated that RV researched in study has a closer genetic relationship with DQ666306, DQ866121, DQ496219, EF556197 which were isolated in 2006 and 2007 from dog, pig and human. This paper accumulated useful materials for further studying molecular epidemiology of cattle RV in China in the future.

CLC Number:

S852.659.5

ZHANG Ke-shan, LIU Yong-jie, KONG Han-jin, SHANG You-jun, LIU Xiang-tao. Identification and Sequence Analysis of Rabies Virus N Gene from Cattle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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Identification and Sequence Analysis of Rabies Virus N Gene from Cattle

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