猪源新型甲型H1N1流感病毒的分离鉴定及遗传进化特点

Abstract

Abstract:

The study was carried out to examine the genetic characteristics of a novel swineorigin influenza A (H1N1) virus isolated from Shandong province. Samples from suspected influenza-infected pigs were collected for viral isolation and identification, and then genetic evolution of HA，NA，PB2，PB1，PA，NP，NS and M of the isolate (A/swine/Shandong/07/2011) was analyzed and compared with the related influenza viruses. The results showed that sequences of 8 fragments of the isolated virus revealed >99% nucleotide identity with A/H1N1(2009) prototype strain, and its HA cleavage and receptor-binding sites were PSIQSR↓GLFGAI and 190D, 225 D, respectively, which were identifical with A/H1N1(2009) . However, compared with HA protein of A/H1N1(2009), mutation Q226R occurred in receptor binding site of the isolate. In conclusion, the results of this study provided significant information for further research on molecular evolution of novel swine-origin influenza A (H1N1) virus.

CLC Number:

S852.659.5

ZHANG Hong-na, MIAO Zeng-min, LI Xin, WANG Hao, ZHOU Yu-fa, XIA Xian-zhu, CHAI Tong-jie. Isolation, Identification and Phylogenetic Analysis of a Novel Swine-originInfluenza A (H1N1) Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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Isolation, Identification and Phylogenetic Analysis of a Novel Swine-originInfluenza A (H1N1) Virus

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