Abstract:

This experiment was conducted to analyze the pathogenic microorganisms in the intestines of dead white feather broilers, clarify the cause of death of white feather broilers, and provide new ideas for scientific disease prevention and treatment of white feather broilers. Intestinal contents of died white feather broilers were collected from a chicken farm, then metagenomic sequencing, sequence splicing, box splitting technology, evolutionary tree analysis, and species annotation were used to identify pathogenic microorganisms in the intestine of white feather broilers. The pathogenic and drug resistance mechanisms of pathogenic microorganisms were elucidated through annotation of genome virulence genes and drug resistance genes. The results showed that the pathogenic microorganism detected in the intestinal contents of diseased white feather broiler chickens was avian pathogenic Escherichia coli. The entire genome sequence of the pathogen was obtained through sequence splicing and box splitting techniques, with a size of 4 998 208 bp and an integrity of 99.23%. This bacterium contains 192 virulence genes, mainly encoding flagella, cilia synthesis related proteins, colonization related proteins, and bacteriocin synthesis related proteins. It contains 88 resistance genes, mainly involving 28 types of antibiotic resistance, indicating that the above types of antibiotics have no therapeutic effect on white feather broilers infected with pathogenic Escherichia coli in poultry, ultimately leading to the death of white jade broilers. This study utilizes macro gene sequencing technology to effectively analyze the pathogenic microorganisms, pathogenesis, and drug resistance mechanisms of white feather broilers. It can help breeders understand the etiology in the early stages of white feather broiler disease, and provide targeted guidance for scientific medication to reduce economic losses.

Key words: White feather broiler, intestinal tract, pathogenic bacteria, metagenomic sequencing, microbiota