玉米赤霉烯酮对山羊生长性能、胃肠道发酵模式和菌群结构的影响研究

doi:10.11843/j.issn.0366-6964.2023.03.023

Abstract

Abstract: This experiment was conducted to investigate the effects of zearalenone (ZEA) addition with different dosages on growth performance, gastrointestinal fermentation pattern and bacterial community structure of Xiangdong black goats. Twenty-four female Xiangdong black goats with similar body weight ((12.82±2.03) kg) were randomly divided into three groups and fed with 0, 100 and 500 μg·kg^-1DM ZEA diet, respectively. The pre-feeding period lasted for one week, and the formal period was kept for four weeks. At the end of the experimental period, all the experimental animals were slaughtered, rumen and colon digesta were collected, and microbial fermentation products were detected by liquid chromatography. DNA of the rumen and colon digesta was extracted and Illumina-Nova sequencing technology was used to analyze the structure of rumen and colon microflora. Compared with the control group, the final body weight, total gain, average daily gain, and dry matter intake of goats were not affected by 100 or 500 μg·kg^-1DM ZEA addition (P>0.05). 500 μg·kg^-1DM ZEA addition decreased the molar percentage of valerate in colon digesta (P<0.05). Compared to the control group, addition of low dosage of ZEA significantly decreased the Shannon index and Simpson index (P<0.05), and addition of high dosage of ZEA significantly decreased the Simpson index and Shannon’s evenness of rumen bacteria (P<0.05). High dosage of ZEA significantly decreased the relative abundance of Firmicutes in the rumen bacteria (P=0.04). The addition of low dosage of ZEA significantly decreased the relative abundance of Actinobacteriota (P=0.04), and the addition of high dosage of ZEA significantly decreased the relative abundance of Spirochaetota in the colon bacteria(P=0.04).In conclusion, dietary 100 or 500 μg·kg^-1DM ZEA did not affect the growth performance and rumen fermentation parameters, but decreased the α diversity and evenness of rumen bacteria. The low-dose ZEA addition affected the relative abundance of Actinobacteriota which was the dominant bacteria in the colon, and the high-dose ZEA addition affected the relative abundance of Firmicutes which was the dominant bacteria in the rumen and Spirochaetota which was the dominant bacteria in the colon of goats.

Key words: zearalenone, goat, growth performance, volatile fatty acid, 16S rRNA sequencing, microbiota diversity

CLC Number:

YAN Qiongxian, CHEN Wenxun, HUI Haoyang, PENG Can, TANG Shaoxun, ZHOU Xiaoling, TAN Zhiliang. Effects of Zearalenone on Growth Performance, Gastrointestinal Fermentation and Microbiota Community Structure of Goats[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1109-1123.

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Effects of Zearalenone on Growth Performance, Gastrointestinal Fermentation and Microbiota Community Structure of Goats

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