瘤胃微生物移植及应用研究进展

doi:10.11843/j.issn.0366-6964.2023.05.003

Abstract

Abstract: The microorganisms habituated in rumen are essential for rumen development, nutrient digestion, and host physiological health. Rumen microbiota transplantation (RMT) refers to transplant specific donor rumen microbiota and their metabolites to the recipient for improving host functional status, production efficiency and product quality, and treating some gastrointestinal diseases of ruminants, by remodeling microflora or restoring microbiome. This article reviewed the development history, application, safety and potential mechanism of RMT, and further elaborated the roles of RMT in revealing the characteristics or functions of rumen microbiota, regulating the balance of microflora and host metabolism, improving growth performance, treating disease, which provided references for the research and application of RMT.

Key words: rumen microbiota transplantation, microbiome manipulation, microbiota remodeling, microbiome restoration, ruminants, safety

CLC Number:

S816.3

ZHAO Wei, Mahmoud M. Abdelsattar, CHAI Jianmin, WANG Xin, DIAO Qiyu, ZHANG Naifeng. Research Progress of Rumen Microbiota Transplantation and Its Application[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1792-1803.

References 93

[1]	WEIMER P J.Redundancy,resilience,and host specificity of the ruminal microbiota:Implications for engineering improved ruminal fermentations[J]. Front Microbiol,2015,6:296.
[2]	NA S W,GUAN L L.Understanding the role of rumen epithelial host-microbe interactions in cattle feed efficiency[J]. Anim Nutr,2022,10:41-53.
[3]	HUWS S A,CREEVEY C J,OYAMA L B,et al.Addressing global ruminant agricultural challenges through understanding the rumen microbiome:Past,present,and future[J]. Front Microbiol,2018,9:2161.
[4]	HU F,XUE Y F,GUO C Z,et al.The response of ruminal fermentation,epithelium-associated microbiota,and epithelial barrier function to severe feed restriction in pregnant ewes[J]. J Anim Sci,2018,96(10):4293-4305.
[5]	MYER P R,FREETLY H C,WELLS J E,et al.Analysis of the gut bacterial communities in beef cattle and their association with feed intake,growth,and efficiency[J]. J Anim Sci,2017,95(7):3215-3224.
[6]	SHABAT S K B,SASSON G,DORON-FAIGENBOIM A,et al.Specific microbiome-dependent mechanisms underlie the energy harvest efficiency of ruminants[J]. ISME J,2016,10(12):2958-2972.
[7]	MAO S Y,HUO W J,ZHU W Y.Microbiome-metabolome analysis reveals unhealthy alterations in the composition and metabolism of ruminal microbiota with increasing dietary grain in a goat model[J]. Environ Microbiol,2016,18(2):525-541.
[8]	MORGAVI D P,RATHAHAO-PARIS E,POPOVA M,et al.Rumen microbial communities influence metabolic phenotypes in lambs[J]. Front Microbiol,2015,6:1060.
[9]	KHAFIPOUR E,LI S C,PLAIZIER J C,et al.Rumen microbiome composition determined using two nutritional models of subacute ruminal acidosis[J]. Appl Environ Microbiol,2009,75(22):7115-7124.
[10]	SHI W B,MOON C D,LEAHY S C,et al.Methane yield phenotypes linked to differential gene expression in the sheep rumen microbiome[J]. Genome Res,2014,24(9):1517-1525.
[11]	MCALLISTER T A,BEAUCHEMIN K A,ALAZZEH A Y,et al.Review:The use of direct fed microbials to mitigate pathogens and enhance production in cattle[J]. Can J Anim Sci,2011,91(2):193-211.
[12]	DEPETERS E J,GEORGE L W.Rumen transfaunation[J]. Immunol Lett,2014,162(2):69-76.
[13]	DE GROOT P F,FRISSEN M N,DE CLERCQ N C,et al.Fecal microbiota transplantation in metabolic syndrome:History, present and future[J]. Gut Microbes,2017,8(3):253-267.
[14]	BRAG S,HANSEN H J.Treatment of ruminal indigestion according to popular belief in Sweden[J]. Rev Sci Tech,1994, 13(2):529-535.
[15]	SINGH R,LANGAR P N,BHATIA I S.Effect of the exchange of rumen contents in the buffalo and zebu on the number of Oscillospira guillermondii[J]. Indian J Vet Sci Anim Husb,1968,38:689-692.
[16]	WAYMACK L B.Effect of feeding lyophilized rumen contents on adaptation to urea diet by lambs[J]. J Anim Sci,1976, 43(3):712-714.
[17]	ZHONG R Z,SUN H X,LI G D,et al.Effects of inoculation with rumen fluid on nutrient digestibility,growth performance and rumen fermentation of early weaned lambs[J]. Livest Sci,2014,162:154-158.
[18]	ABO-DONIA F M,GHADA S I,NADI S,et al.Effect of inoculating new born lambs with fresh or lyophilized rumen fluid on rumen activity and lamb performance[J]. J Am Sci,2011,7(9):409-422.
[19]	YU S B,SHI W B,YANG B,et al.Effects of repeated oral inoculation of artificially fed lambs with lyophilized rumen fluid on growth performance,rumen fermentation,microbial population and organ development[J]. Anim Feed Sci Technol,2020,264:114465.
[20]	PALMA-HIDALGO J M,JIMENEZ E,POPOVA M,et al.Inoculation with rumen fluid in early life accelerates the rumen microbial development and favours the weaning process in goats[J]. Anim Microbiome,2021,3(1):11.
[21]	HUANG S,ZHENG G,MEN H K,et al.The response of fecal microbiota and host metabolome in dairy cows following rumen fluid transplantation[J]. Front Microbiol,2022,13:940158.
[22]	MUSCATO T V,TEDESCHI L O,RUSSELL J B.The effect of ruminal fluid preparations on the growth and health of newborn,milk-fed dairy calves[J]. J Dairy Sci,2002,85(3):648-656.
[23]	PARK T,CERSOSIMO L M,LI W L,et al.Pre-weaning ruminal administration of differentially-enriched,rumen-derived inocula shaped rumen bacterial communities and co-occurrence networks of post-weaned dairy calves[J]. Front Microbiol,2021, 12:625488.
[24]	CERSOSIMO L M,RADLOFF W,ZANTON G I.Microbial inoculum composition and pre-weaned dairy calf age alter the developing rumen microbial environment[J]. Front Microbiol,2019,10:1651.
[25]	PARK T,CERSOSIMO L M,RADLOFF W,et al.The rumen liquid metatranscriptome of post-weaned dairy calves differed by pre-weaning ruminal administration of differentially-enriched,rumen-derived inocula[J]. Anim Microbiome,2022,4(1):4.
[26]	LIU K Z,ZHANG Y D,YU Z T,et al.Ruminal microbiota-host interaction and its effect on nutrient metabolism[J]. Anim Nutr,2021,7(1):49-55.
[27]	CHEN X D,YAN F,LIU T,et al.Ruminal microbiota determines the high-fiber utilization of ruminants:Evidence from the ruminal microbiota transplant[J]. Microbiol Spectr,2022,10(4):e00446-22.
[28]	CHEN X D,SU X D,LI J L,et al.Real-time monitoring of ruminal microbiota reveals their roles in dairy goats during subacute ruminal acidosis[J]. npj Biofilms Microbiomes,2021,7(1):45.
[29]	ZHOU M,PENG Y J,CHEN Y H,et al.Assessment of microbiome changes after rumen transfaunation:implications on improving feed efficiency in beef cattle[J]. Microbiome,2018,6(1):62.
[30]	IMAI S,MATSUMOTO M,WATANABE A,et al.Establishment of a spinated type of Diplodinium rangiferi by transfaunation of the rumen ciliates of Japanese sika deer (Cervus nippon centralist to the Rumen of Two Japanese Shorthorn Calves (Bos taurus taurus)[J]. J Eukaryot Microbiol,2002,49(1):38-41.
[31]	WILLIAMS A G,WITHERS S E.Changes in the rumen microbial population and its activities during the refaunation period after the reintroduction of ciliate protozoa into the rumen of defaunated sheep[J]. Can J Microbiol,1993,39(1):61-69.
[32]	SANTOS B P,BESSEGATTO J A,ALFIERI A A,et al.Transfaunation of the ruminal fluid from cows alters ruminal microbiota structure but not dominant protozoa in healthy sheep[J]. Small Ruminant Res,2021,194:106283.
[33]	STEINER S,LINHART N,NEIDL A,et al.Evaluation of the therapeutic efficacy of rumen transfaunation[J]. J Anim Physiol Anim Nutr,2020,104(1):56-63.
[34]	JASMIN B H,BOSTON R C,MODESTO R B,et al.Perioperative ruminal pH changes in domestic sheep (Ovis aries) housed in a biomedical research setting[J]. J Am Assoc Lab Anim Sci,2011,50(1):27-32.
[35]	CONRAD H R,HIBBS J W,POUNDEN W D,et al.The effect of rumen inoculations on the digestibility of roughages in young dairy calves[J]. J Dairy Sci,1950,33(8):585-592.
[36]	RICO D E,YING Y,CLARKE A R,et al.The effect of rumen digesta inoculation on the time course of recovery from classical diet-induced milk fat depression in dairy cows[J]. J Dairy Sci,2014,97(6):3752-3760.
[37]	WEIMER P J,COX M S,DE PAULA T V,et al.Transient changes in milk production efficiency and bacterial community composition resulting from near-total exchange of ruminal contents between high-and low-efficiency Holstein cows[J]. J Dairy Sci,2017,100(9):7165-7182.
[38]	ARIK H D,GULSEN N,HAYIRLI A,et al.Efficacy of Megasphaera elsdenii inoculation in subacute ruminal acidosis in cattle[J]. J Anim Physiol Anim Nutr (Berl),2019,103(2):416-426.
[39]	QUIRK M F,BUSHELL J J,JONES R J,et al.Live-weight gains on leucaena and native grass pastures after dosing cattle with rumen bacteria capable of degrading DHP,a ruminal metabolite from leucaena[J]. J Agric Sci,1988,111(1):165-170.
[40]	DA SILVA L C A,PESSOA D A D N,LOPES J R G,et al.Resistance transferance of Amorimia septentrionalis poisoning in goats by ruminal transferance fluid from goats with induced resistance by the inoculation of sodium monofluoroacetate-degrading bacteria[J]. Ciencia Rural,2015,45(12):2218-2222.
[41]	RAGER K D,GEORGE L W,HOUSE J K,et al.Evaluation of rumen transfaunation after surgical correction of left-sided displacement of the abomasum in cows[J]. J Am Vet Med Assoc,2004,225(6):915-920.
[42]	LIU J H,LI H W,ZHU W Y,et al.Dynamic changes in rumen fermentation and bacterial community following rumen fluid transplantation in a sheep model of rumen acidosis:implications for rumen health in ruminants[J]. FASEB J,2019,33(7):8453-8467.
[43]	JI S K,JIANG T,YAN H,et al.Ecological restoration of antibiotic-disturbed gastrointestinal microbiota in foregut and hindgut of cows[J]. Front Cell Infect Microbiol,2018,8:79.
[44]	LEO-PENU C L O,FITZPATRICK L A,ZERBY H N,et al.Treating Bos indicus bulls with rumen transfaunation after 24 hours of transportation does not replete muscle glycogen[J]. Anim Prod Sci,2015,10(56):1738-1744.
[45]	VAN METRE D C,CALLAN R J,HOLT T N,et al.Abdominal emergencies in cattle[J]. Vet Clin North Am Food Anim Pract, 2005, 21(3):655-696.
[46]	BU D P,ZHANG X,MA L,et al.Repeated inoculation of young calves with rumen microbiota does not significantly modulate the rumen prokaryotic microbiota consistently but decreases diarrhea[J]. Front Microbiol,2020,11:1403.
[47]	张鑫.灌服瘤胃液对犊牛生长性能及胃肠道发育的影响[D].扬州:扬州大学,2017.ZHANG X.Effects of rumen fluid on growth performance and gastrointestinal development of calves[D].Yangzhou:Yangzhou University,2017.(in Chinese)
[48]	YÁÑEZ-RUIZ D R,ABECIA L,NEWBOLD C J.Manipulating rumen microbiome and fermentation through interventions during early life:a review[J]. Front Microbiol,2015,6:1133.
[49]	YIN X,JI S,DUAN C,et al.Rumen fluid transplantation affects growth performance of weaned lambs by altering gastrointestinal microbiota,immune function and feed digestibility[J]. Animal,2021,15(1):100076.
[50]	MU Y Y,QI W P,ZHANG T,et al.Changes in rumen fermentation and bacterial community in lactating dairy cows with subacute rumen acidosis following rumen content transplantation[J]. J Dairy Sci,2021,104(10):10780-10795.
[51]	CERSOSIMO L M,RADLOFF W,ZANTON G I.Microbial inoculum composition and pre-weaned dairy calf age alter the developing rumen microbial environment[J]. Front Microbiol,2019,10:1651.
[52]	PEÑA-CEARRA A,BELANCHE A,GONZALEZ-LOPEZ M,et al.Peripheral blood mononuclear cells (PBMC) microbiome is not affected by colon microbiota in healthy goats[J]. Anim Microbiome,2021,3(1):28.
[53]	HITCH T C A,HALL L J,WALSH S K,et al.Microbiome-based interventions to modulate gut ecology and the immune system[J]. Mucosal Immunol,2022,15(6):1095-1113.
[54]	HAN L L,XUE W C,CAO H W,et al.Comparison of rumen fermentation parameters and microbiota of yaks from different altitude regions in Tibet,China[J]. Front Microbiol,2022,12:807512.
[55]	DE BARBIERI I,HEGARTY R S,SILVEIRA C,et al.Programming rumen bacterial communities in newborn Merino lambs[J]. Small Ruminant Res,2015,129:48-59.
[56]	FRIEDMAN N,JAMI E,MIZRAHI I.Compositional and functional dynamics of the bovine rumen methanogenic community across different developmental stages[J]. Environ Microbiol,2017,19(8):3365-3373.
[57]	BELANCHE A,PALMA-HIDALGO J M,NEJJAM I,et al. In vitro assessment of the factors that determine the activity of the rumen microbiota for further applications as inoculum[J]. J Sci Food Agric,2019,99(1):163-172.
[58]	SHEN J S,CHAI Z,SONG L J,et al.Insertion depth of oral stomach tubes may affect the fermentation parameters of ruminal fluid collected in dairy cows[J]. J Dairy Sci,2012,95(10):5978-5984.
[59]	LI M,PENNER G B,HERNANDEZ-SANABRIA E,et al.Effects of sampling location and time,and host animal on assessment of bacterial diversity and fermentation parameters in the bovine rumen[J]. J Appl Microbiol,2009,107(6):1924-1934.
[60]	WEIMER P J.Redundancy,resilience,and host specificity of the ruminal microbiota:implications for engineering improved ruminal fermentations[J]. Front Microbiol,2015,6:296.
[61]	MALMUTHUGE N,GUAN L L.Understanding host-microbial interactions in rumen:searching the best opportunity for microbiota manipulation[J]. J Anim Sci Biotechnol,2017,8:8.
[62]	RIBEIRO G O,OSS D B,HE Z X,et al.Repeated inoculation of cattle rumen with bison rumen contents alters the rumen microbiome and improves nitrogen digestibility in cattle[J]. Sci Rep,2017,7(1):1276.
[63]	HUANG W Q,CUI K,HAN Y,et al.Long term effects of artificial rearing before weaning on the growth performance, ruminal microbiota and fermentation of fattening lambs[J]. J Integr Agr,2022,21(4):1146-1160.
[64]	CHAI J M,DIAO Q Y,WANG H C,et al.Effects of weaning age on growth,nutrient digestibility and metabolism,and serum parameters in Hu lambs[J]. Anim Nutr,2015,1(4):344-348.
[65]	BHARANIDHARAN R,LEE C H,THIRUGNANASAMBANTHAM K,et al.Feeding systems and host breeds influence ruminal fermentation,methane production,microbial diversity and metagenomic gene abundance[J]. Front Microbiol,2021, 12:701081.
[66]	SPANGHERO M,CHIARAVALLI M,COLOMBINI S,et al.Rumen inoculum collected from cows at slaughter or from a continuous fermenter and preserved in warm,refrigerated,chilled or freeze-dried environments for in vitro tests[J]. Animals, 2019,9(10):815.
[67]	MOULD F L,KLIEM K E,MORGAN R,et al. In vitro microbial inoculum:a review of its function and properties[J]. Anim Feed Sci Technol,2005,123-124:31-50.
[68]	COX M S,DEBLOIS C L,SUEN G.Assessing the response of ruminal bacterial and fungal microbiota to whole-rumen contents exchange in dairy cows[J]. Front Microbiol,2021,12:665776.
[69]	COLE N A.Effects of animal-to-animal exchange of ruminal contents on the feed intake and ruminal characteristics of fed and fasted lambs[J]. J Anim Sci,1991,69(4):1795-1803.
[70]	BELANCHE A,PALMA-HIDALGO J M,NEJJAM I,et al.Inoculation with rumen fluid in early life as a strategy to optimize the weaning process in intensive dairy goat systems[J]. J Dairy Sci,2020,103(6):5047-5060.
[71]	YU S B,ZHANG G Y,LIU Z B,et al.Repeated inoculation with fresh rumen fluid before or during weaning modulates the microbiota composition and co-occurrence of the rumen and colon of lambs[J]. BMC Microbiol,2020,20(1):29.
[72]	王佳堃,杨斌,黄开朗.幼龄反刍动物消化道微生物演替与消化道发育及其调控[J].动物营养学报,2020, 32(10):4697-4707.WANG J K,YANG B,HUANG K L.Regulation of microbial succession and gastrointestinal development in young ruminants[J]. Chinese Journal of Animal Nutrition,2020,32(10):4697-4707.(in Chinese)
[73]	ABDELSATTAR M M,VARGAS-BELLO-PÉREZ E,ZHUANG Y M,et al.Impact of dietary supplementation of β-hydroxybutyric acid on performance,nutrient digestibility,organ development and serum stress indicators in early-weaned goat kids[J]. Anim Nutr,2022,9:16-22.
[74]	GÓRKA P,KOWALSKI Z M,ZABIELSKI R,et al. Invited review:use of butyrate to promote gastrointestinal tract development in calves[J]. J Dairy Sci,2018,101(6):4785-4800.
[75]	LIANG G X,MALMUTHUGE N,MCFADDEN T B,et al.Potential regulatory role of microRNAs in the development of bovine gastrointestinal tract during early life[J]. PLoS One,2014,9(3):e92592.
[76]	WON M Y,OYAMA L B,COURTNEY S J,et al.Can rumen bacteria communicate to each other[J]. Microbiome, 2020,8(1):23.
[77]	张定然,吴燕,邢小光,等.瘤胃液移植技术及应用的研究进展[J].中国畜牧杂志,2021,57(8):28-32.ZHANG D R,WU Y,XING X G,et al.Research progress on the technology and application of rumen fluid transplantion[J]. Chinese Journal of Animal Science,2021,57(8):28-32.(in Chinese)
[78]	DEFILIPP Z,BLOOM P P,SOTO M T,et al.Drug-Resistant E. coli bacteremia transmitted by fecal microbiota transplant[J]. N Engl J Med,2019,381(21):2043-2050.
[79]	焦帅,付域泽,张乃锋.下一代益生菌作用机制及功能特性研究进展[J].动物营养学报,2022,34(8):4836-4846.JIAO S,FU Y Z,ZHANG N F.Progress on mechanism and functional characteristics of next generation probiotics[J]. Chinese Journal of Animal Nutrition,2022,34(8):4836-4846.(in Chinese)
[80]	HU J,CHEN L L,TANG Y M,et al.Standardized preparation for fecal microbiota transplantation in pigs[J]. Front Microbiol, 2018,9:1328.
[81]	QAZI T,AMARATUNGA T,BARNES E L,et al.The risk of inflammatory bowel disease flares after fecal microbiota transplantation:systematic review and meta-analysis[J]. Gut Microbes,2017,8(6):574-588.
[82]	FANIYI T O,ADEGBEYE M J,ELGHANDOUR M M M Y,et al.Role of diverse fermentative factors towards microbial community shift in ruminants[J]. J Appl Microbiol,2019,127(1):2-11.
[83]	WEIMER P J,STEVENSON D M,MANTOVANI H C,et al.Host specificity of the ruminal bacterial community in the dairy cow following near-total exchange of ruminal contents[J]. J Dairy Sci,2010,93(12):5902-5912.
[84]	NAVARRE C B,BAIRD A N,PUGH D G.Diseases of the gastrointestinal system[M]//PUGH D G,BAIRD A N.Sheep and Goat Medicine.2nd ed.Amsterdam:Elsevier,2012:71-105.
[85]	ASRAT M,MANOHAR B M,MELKAMU S.Clinical and rumen fluid evaluation of ruminal disorders in cattle[J]. J Anim Res, 2015,5(2):359-372.
[86]	KRAUSE K M,OETZEL G R.Understanding and preventing subacute ruminal acidosis in dairy herds:a review[J]. Anim Feed Sci Technol,2006,126(3-4):215-236.
[87]	ZHANG T,LU G C,ZHAO Z,et al.Washed microbiota transplantation vs.manual fecal microbiota transplantation:clinical findings,animal studies and in vitro screening[J]. Protein Cell,2020,11(4):251-266.
[88]	ZHAO W,ABDELSATTAR M M,WANG X,et al. In vitro modulation of rumen fermentation by microbiota from the recombination of rumen fluid and solid phases[J]. Microbiol Spectr,2023,11(1):e03387-22.
[89]	CHAI J M,LV X K,DIAO Q Y,et al.Solid diet manipulates rumen epithelial microbiota and its interactions with host transcriptomic in young ruminants[J]. Environ Microbiol,2021,23(11):6557-6568.
[90]	马涛,张乃锋,屠焰,等.羔羊瘤胃微生物区系建立、发展与调控技术研究进展[J].动物营养学报,2020, 32(10):4733-4742.MA T,ZHANG N F,TU Y,et al.Research advance in establishment,development and regulation technology of rumen microbiota in lambs[J]. Chinese Journal of Animal Nutrition,2020,32(10):4733-4742.(in Chinese)
[91]	WALTER J,ARMET A M,FINLAY B B,et al.Establishing or exaggerating causality for the gut microbiome:lessons from human microbiota-associated rodents[J]. Cell,2020,180(2):221-232.
[92]	WALTER J,MALDONADO-GÓMEZ M X,MARTÍNEZ I.To engraft or not to engraft:an ecological framework for gut microbiome modulation with live microbes[J]. Curr Opin Biotechnol,2018,49:129-139.
[93]	LAVELLE A,SOKOL H.Understanding and predicting the efficacy of FMT[J]. Nat Med,2022,28(9):1759-1760.

Research Progress of Rumen Microbiota Transplantation and Its Application

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 93

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	YU Shixiong, WEI Lingyun, XU Tiantian, JIAO Jinzhen, JIANG Linshu, HE Zhixiong. Research Progress of Intestinal Microbial Colonization Pattern in Young Ruminants and Its Nutritional Regulation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2701-2707.
[2]	WANG Yu, GAO Yueyi, GAO Jinyuan, LIU Weijie, XU Huilin, XUE Qinghong. Establishment of Reverse Genetics System of Peste des petits Ruminants Virus Clone9 Strain [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2956-2963.
[3]	FANG Yuan, HOU Qiaodi, XIANG Chaohui, ZHAO Hongyi, QI Xuefeng. Regulatory Effects of IFITM3 on Proliferation of Peste des Petits Ruminants Virus (PPRV) in Goat Endometrial Epithelial Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2200-2207.
[4]	WANG Yiqun, LIU Zupei, LI Yating, ZHANG Haisen, LI Dan, JIN Yaping, CHEN Huatao. The Dairy Cow NR1D1 Gene’s Eukaryotic Expression Vector Construction, Expression Profile and Its Ovarian Localization [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 133-145.
[5]	PAN Chunrong, DENG Ruixue, HU Linjie, ZHU Xueliang, SUN Yuefeng, WANG Guirong, MENG Xuelian. Effect of Histone Deacetylase Inhibitors on Peste Des Petits Ruminants Virus Replication [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2307-2316.
[6]	ZHAO Xu, LING Yuzhao, WANG Jianhua, WEI Lingyun, JIAO Jinzhen, HE Zhixiong. Research Progress on Rumen Microbial Colonization and Nutritional Regulation of Young Ruminants [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3296-3304.
[7]	TONG Panpan, ZHANG Mengmeng, CHEN Wenxia, LIU Luyao, ZHANG Ling, TANG Xuelin, SU Zhanqiang, XIE Jinxin. Phylogenetic Clustering, Serotype, Virulence Genes and Drug Resistance Analysis of Shiga Toxin-producing Escherichia coli from Cattle, Sheep and Camel in Xinjiang [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(12): 3660-3668.
[8]	QIAN Bang, LI Yanmin, ZHU Xueliang, ZHANG Xueyan, Niyokwishimira Alfred, DOU Yongxi, ZHANG Zhidong. Establishment of an iELISA Method for Detection of Antibody againist Peste des Petits Ruminants Virus Based on H Protein Epitope Peptide [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(1): 144-153.
[9]	YANG Ailing, LI Guangdong, WU Hao, JI Pengyun, ZHANG Lu, ZHANG Jinlong, ZHANG Xiaosheng, WUSIMAN Abulizi, LIAN Zhengxing, LIU Guoshi. Study of Biosafety Evaluation on Melatonin Synthase AANAT/ASMT Overexpressed Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(7): 1563-1572.
[10]	ZHUANG Yimin, DIAO Qiyu, ZHANG Naifeng. Metabolism and Regulation Mechanism of Beta-hydroxybutyric Acid in Ruminal Epithelium Cells of Young Ruminants [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(4): 660-669.
[11]	WANG Xinhua, ZHANG Xingxing, WANG Limin, HUANG Xin, HAN Mengli, ZHANG Yiyuan, GUO Yanhua, TANG Hong, HE Yanhua, ZHONG Fagang, ZHOU Ping. Differences of the Intestinal Microbial Flora Diversity and Composition in IGF-1 Transgenic Superfine Wool Sheep and Non-transgenic Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(10): 2387-2402.
[12]	CHU Yunxin, CHEN Yan, MA Yuqing, ZENG Wei, CHEN Songrui, ZHAO Zhiqian, WANG Jingyu, QI Xuefeng. Effect of Exosomes Derived from PPRV Vaccine Strain N75-1 Infected Cells on the Expression of SLAM in Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(10): 2500-2508.
[13]	CHEN Xia, HE Zhaoxiang, XU Guiyun, ZHENG Jiangxia. Research Progress on Eggshell Cuticle [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(1): 18-26.
[14]	WU Jinyan, TIAN Hong, MENG Xuelian, HUI Xiaoting, WANG Yaojie, GUAN Yuhua, SHANG Youjun, LIU Yongsheng, ZHANG Zhidong. Establishment of Vero/SLAM Cell Lines with Stable Expression SLAM to Enhanced PPRV Replication Using the Lentiviral Expression System [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 218-226.
[15]	WANG Yan-xin, XIE Shu-yu, CHEN Dong-mei, WANG Yu-lian, PAN Yuan-hu, PENG Da-peng, TAO Yan-fei, YUAN Zong-hui. Progress in the Application of Electrochemical Immunosensor in Food Safety Detection [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(7): 1334-1342.