基于16S rRNA测序分析高低饲料转化率猪粪便微生物的组成差异

doi:10.11843/j.issn.0366-6964.2024.04.023

Abstract

Abstract: Improving feed conversion efficiency in pigs is currently a key focus of pig breeding efforts. In this study, FCR values of 384 crossbred sows were ranked, and 20 pigs with extreme feed conversion ratio (10 with the highest FCR and 10 with the lowest FCR) were selected to form the HFCR group and LFCR group, respectively. Fecal samples were collected for 16S rRNA gene sequencing analysis. The results showed that the Richness index, Chao1 index, and ACE index of the LFCR group are significantly higher than those of the HFCR group (P < 0.01). The LEfSe analysis was used to identify microorganisms at the genus level with significant differences between the two groups. In the LFCR group, microorganisms with LDA>3 were mainly included Prevotella_1, Treponema_2, Prevotellaceae_NK3B31_group, Eubacterium_coprostanoligenes_group, p-1088-a5_gut_group, Prevotella_9, Akkermansia, Prevotellaceae_UCG_009, Ruminococcaceae_UCG-014, and Ruminococcaceae_NK4A214_group. In the HFCR group, microorganisms with LDA>3 were mainly included Megasphaera, Dialister, Catenibacterium, Pseudobutyrivibrio, Collinsella, Solobacterium, Subdoligranulum, and Streptococcus. The results of Spearman correlation analysis indicated that microorganisms significantly negatively correlated with FCR are mostly associated with short-chain fatty acid production, while microorganisms significantly positively correlated with FCR were mostly potential pathogenic bacteria. This study screened differential microorganisms in feces from pigs with high and low feed conversion rates, also conducted correlation analysis between the abundance of differential microorganisms and feed conversion rate traits, and ultimately identified fecal microorganisms significantly associated with feed conversion rate, thus provided a reference for the selection of intestinal microbial breeding markers for pig feed conversion rate traits.

Key words: feed conversion rate, 16S rRNA, fecal microbiota

CLC Number:

S828.2

ZHANG Shuai, CHEN Kuirong, XU Di, JIANG Shan, WANG Mengying, ZHANG Kun, XU Yupei, LEI Guofeng, ZHANG Zhicheng, GUO Meng, ZHAO Yunxiang, LAN Ganqiu, LIANG Jing. Analysis of Microbial Composition Differences in High and Low Feed Conversion Rates Pig Feces based on 16S rRNA Sequencing[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1605-1614.

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Analysis of Microbial Composition Differences in High and Low Feed Conversion Rates Pig Feces based on 16S rRNA Sequencing

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