鸡滑液囊支原体对常用抗菌药流行病学临界值的建立

doi:10.11843/j.issn.0366-6964.2024.06.041

Abstract

Abstract:

Mycoplasma synoviae (MS) is one of the most detrimental mycoplasmas in the large-scale poultry industry. Meanwhile, the development of antimicrobial resistance to MS is serious currently. In order to understand the current situation of antimicrobial resistance to MS, the research was conducted in reference to the method of establishing epidemiological/wildtype cut-off values (ECOFFs/CO_WT) in CLSI and EUCAST by collecting and summarizing MIC from different regions and sources in recent 5 years after searching relevant literature reports at home and abroad, and then the ECOFFs of various antimicrobial agents to MS were obtained through comprehensive analysis using visual estimation, ECOFFFinder software analysis and Normalized Drug Resistance Interpretation (NRI). Subsequently, the corresponding drug resistance rates were calculated. It showed that the ECOFFs of MS to tylosin, tilmicosin, tylvalosin, doxycycline, oxytetracycline, chlortetracycline, tiamulin, lincomycin, florfenicol and spectinomycin were 1, 0.5, 0.5, 2, 4, 2, 2, 2, 8 and 4 μg·mL^-1 respectively. In absence of sensitive breakpoint of MS in CLSI and ECUAST, the theoretical basis and scientific evidence are provided by this research for the rational use of drugs in clinical practice, development of antimicrobial resistance work for monitoring and reducing it, and also beneficial for the treatment and control of MS.

Key words: Mycoplasma synoviae, epidemiological cut-off values, drug resistance, ECOFFinder software, normalized drug resistance interpretation (NRI)

CLC Number:

S852.62

Ziying ZOU, Anxiong HUANG, Zihan RUAN, Haihong HAO. Establishment on Commonly Used Antimicrobials Epidemiological Cut-off Values of Mycoplasma synoviae[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(6): 2701-2715.

Figures/Tables 5

Table 1

Table 2

Fig. 1

Fig. 2

Table 3

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药物种类 Drug classification	药物名称 Drugs	菌株总数 Number	来源地区 Data source	相关文献 Related literature
大环内酯类	泰乐菌素Tylosin	453	欧洲、亚洲	[26-34]
Macrolides	替米考星Tilmicosin	448	欧洲、亚洲、非洲	[26-34]
	泰万菌素Tylvalosin	238	欧洲	[26, 28, 32-34]
四环素类	多西环素Doxycycline	198	欧洲、亚洲	[27-29, 31]
Tetracyclines	土霉素Oxytetracycline	301	欧洲、亚洲、大洋洲、非洲	[26-29, 31-32, 34]
	金霉素Chlortetracycline	133	欧洲、亚洲、大洋洲、非洲	[26, 32, 34]
氟喹诺酮类	恩诺沙星Enrofloxacin	217	欧洲、亚洲	[28-31]
Fluoroquinolones
截短侧耳素类	泰妙菌素Tiamulin	245	欧洲、亚洲	[26-31]
Pleuromutilins
林可酰胺类	林可霉素Lincomycin	305	欧洲、亚洲、非洲、大洋洲	[26, 31-34]
Lincomamides
氯霉素类	氟苯尼考Florfenicol	321	欧洲、亚洲、非洲、大洋洲	[26, 28, 31-32, 34]
Chloramphenicols
氨基糖苷类	大观霉素Spectinomycin	139	欧洲、亚洲、非洲、大洋洲	[26, 32, 34]
Aminoglycosides	新霉素Neomycin	118	欧洲、亚洲、非洲、大洋洲	[32, 34]

药物 Drugs	MIC₅₀/(μg·mL^-1)	MIC₉₀/(μg·mL^-1)	ECOFF _V_isual /(μg·mL^-1)	ECOFF _ECOFF_inder/(μg·mL^-1)	ECOFF _NRI/(μg·mL^-1)	ECOFF _Final/(μg·mL^-1)	耐药率/% DR
泰乐菌素TYL	0.25	2	1	1	0.25	1	11.0
替米考星TIL	0.5	128	0.5	0.5	4	0.5	46.9
泰万菌素TVL	0.25	0.5	0.5	0.5	0.5	0.5	4.2
多西环素DOX	0.5	2	2	2	2	2	5.6
土霉素OTC	1	2	4	4	8	4	1.4
金霉素CTC	1	4	2	2	2	2	17.3
恩诺沙星ENR	32	32	/	/	/	/	/
泰妙菌素TML	0.25	1	2	2	0.125	2	0.4
林可霉素LCM	1	128	2	2	4	2	18.0
氟苯尼考FFC	2	8	8	8	16	8	0
大观霉素SPT	2	4	4	4	8	4	2.2
新霉素NEM	32	128	128	128	128	/	/

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Establishment on Commonly Used Antimicrobials Epidemiological Cut-off Values of Mycoplasma synoviae

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MIC/(μg·mL^-1)	抗菌药物Antimicrobial agents
MIC/(μg·mL^-1)	TYL	TIL	TVL	DOX	OTC	CTC	ENR	TML	LCM	FFC	SPT	NEM
0.004	3^a							3^a
0.008								9
0.016	14	7						26
0.031	43	7						14
0.063	50	10	6	4^a	1			14^a
0.125	28	14	2	2	2			15
0.25	201^a	145^a	200^a	20	45^a	22^a		57	40^a	24^a	3^a
0.5	45	55	20	89	57	40		78	69	7	10
1	19	56	1	59	108	31	4	20	120	88	39
2	32	27		13	64	17	11	8	21	97	67
4	4	19	8	8	19	10	17	0	12	62	17	2
8	3	19	1	3	5	4	20	0	0	43	3	9
16	3	13				9^b	36	0	0			23
32	4	12					129^b	1	0			52
64	2	15^b							2^b			17
128	2^b	48^b							41			15^b
256		1^b
总计Total	453	448	238	198	348	133	217	245	305	321	139	118