ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2012, Vol. 43 ›› Issue (6): 965-971.doi:

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Cloning and Sequence Analysis of the gB, gC, gD Genes of IBRV from Inner Mongolia

HU Wen-bing1,2,GUAN Ping-yuan1,4*,HAO Rui-feng1,ZHAO Peng-wei1,Wurihan3   

  1. 1. College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;2. Xinjiang Bazhou Animal Health Inspection Institute, Korla 841000, China; 3. Center of Prevent and Control of Animal Disease of Inner Mongolia, Hohhot 010010, China; 4. Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture, Hohhot 010018, China
  • Received:2011-09-05 Online:2012-06-25 Published:2012-06-25
  • Contact: GUAN Pingyuan

Abstract:

The gB, gC and gD genes of Inner Mongolia IBRV strain were cloned and sequenced. The results showed that the gB, gC and gD genes were 2 850, 1 723 and 1 559 bp, respectively. And the 3 genes contained their corresponding open reading frames (ORF) which encoding 933, 508 and 417 amino acids, respectively. The gB, gC and gD genes of Inner Mongolia strain shared 94.9% to 99.9% homology in nucleotide with others strain from GenBank, and shared 90.3% to 99.7% homology in deduced amino acid sequence by the sequences and phylogenetic analysis. The gB, gC and gD genes were highly conserved among IBRV strains. Phylogenetic tree of IBRV revealed that the Inner Mongolia strain was consistently located in the same lineages with Sweden strain.

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