基于拉曼光谱分析猪卵母细胞玻璃化冷冻前后变化

doi:10.11843/j.issn.0366-6964.2025.10.020

Abstract

Abstract:

This study aimed to investigate molecular structural changes in porcine mature oocytes before and after vitrification using Raman spectroscopy. Ovaries were collected from pigs at a slaughterhouse, and cumulus-oocyte complexes were isolated in the laboratory. After in vitro maturation, fresh oocytes and vitrified oocytes were collected, respectively. Laser confocal Raman spectrometer was used to obtain the Raman spectrum information of fresh oocytes and vitrified oocytes. The molecular structure changes of oocytes before and after vitrification were analyzed after quality control of the data. The results indicated that, compared to fresh oocytes, vitrified oocytes exhibited reduced carbohydrate components such as N-acetylgalactosamine within the carbohydrate signal range of 1 020-1 140 cm^-1. In the amide Ⅲ band signal range (1 230-1 300 cm^-1), β-sheet content increased markedly while α-helix structures decreased. Within the lipid signal range (2 800-3 100 cm^-1), the content of long-chain fatty acids decreased, while the content of branched-chain fatty acids increased. In summary, vitrification alters the secondary protein structure, fatty acid composition and other key components like N-acetylgalactosamine in porcine oocytes. These changes result in multiple cryodamage effects, including protein structural damage, altered lipid composition, and abnormal carbohydrate metabolism.

Key words: porcine, oocyte, Raman spectrum, vitrification

CLC Number:

S828.3

CHEN Siying, LI Kang, SUN Yawen, LENG Xuan, WANG Dong, PANG Yunwei. Analysis of Changes of Porcine Oocytes before and after Vitrification Based on Raman Spectroscopy[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(10): 4998-5006.

Figures/Tables 6

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Table 1

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峰/(cm^-1) Peak	核酸 DNA/RNA	蛋白质 Protein	脂质 Lipid	碳水化合物 Carbohydrate	其他成分 Others
944		C-C BK str
1 004		sym ring br Phe
1 097	sym str PO^2-	C-N str			sym str PO₄^3-
1 128		C-N str		C-O str
1 020~1 140		C-N str		C-O str, C-O-H br
1 262	T, A	Amide Ⅲ	═C-H ben
1 303		CH₃/CH₂ twi, ben	CH₂ twi, ben
1 340			C-H def
1 444		CH def	CH def	CH def
1 659		Amide Ⅰ	C═C str
1 747			C═O str^[25]
2 820~3 000		sym str CH₃/CH₂	sym str CH₃/CH₂

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Analysis of Changes of Porcine Oocytes before and after Vitrification Based on Raman Spectroscopy

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