Table 1

Primers for qRT-PCR"

Fig. 1

Effects of glycine supplementation on survival, maturation and development of mink oocytes during vitrification, thawing and IVM A. Representative images of oocytes were collected 24 h after IVM. The arrow indicates dead cells, but not all dead cells. Scale: 100 μm. B. Impact of varying glycine concentrations (0, 1, 5, 10 mmol·L-1) on post-thaw oocyte survival rate. C. Statistical charts of the survival rate of oocytes collected in 3 groups. D. Characteristic representative images of the nuclei of oocytes at different stages of development. Scale bar: 20 μm. E. Statistical analysis of 4 major stages of oocyte nuclear development in 3 groups"

Fig. 2

Effects of glycine supplementation during vitrification, thawing and IVM on mitochondrial distribution and activity of mink oocytes at GV stage A. Oocyte functional mitochondrial immunofluorescence staining (red), DAPI (blue). Merge. Red and blue overlap. Scale bar: 20 μm. B. Percentage of oocytes with normal mitochondrial distribution. C. Oocyte mitochondrial activity in different experimental treatments"

Fig. 3

Effects of glycine supplementation during vitrification, thawing and IVM on ROS levels in mature oocytes at GV stage of mink A. ROS staining representative images of oocytes in the experimental group (green). Scale bar: 100 μm. B. Statistical plots of relative fluorescence intensity of ROS staining of oocytes under different treatments"

Fig. 4

Effect of glycine supplementation during vitrification, thawing, and IVM on the migration of cortical granules (CGs) in mink oocytes A. Representative images of CGs distribution in oocytes collected after maturation in each experimental group. Green indicate cortical granules (CGs); Blue indicate chromatin; Merge indicate green and blue overlap. Scale bar: 20 μm. B. Percentage of oocytes with normal CGs distribution in each experimental group"

Fig. 5

Effect of glycine supplementation during vitrification, thawing and IVM on oocyte apoptosis of mink A. Representative images of early apoptosis labeled with Annexin-V in each experimental group. Green indicate Annexin-V dyed. Scale: 100 μm. B. Relative fluorescence intensity of Annexin-V staining of oocytes in each experimental group. C. The relative transcription levels of genes involved in oocyte apoptosis in each experimental group, with GAPDH as the house-keeping gene"

Fig. 6

Effects of glycine supplementation during vitrification, thawing and IVM on epigenetic modification of mink oocytes A. Representative images of the distribution of H3K9me3 in oocytes collected after maturity in each experimental group. Green indicate H3K9me3; Blue indicate chromatin; Merge indicate green and blue overlap. Scale bar: 20 μm. B. The relative fluorescence intensity of H3K9me3 in each experimental group. C. Relative transcription levels of DNA methyltransferase 1(DNMT1) in oocytes of each experimental group, with GAPDH acting as the house-keeping gene"