猪瘟病毒C株表位突变毒株的构建及拯救

doi:10.11843/j.issn.0366-6964.2024.02.027

Abstract

Abstract: An infectious clone was constructed by mutating the WH303 site of CSFV C strain to provide candidate strains for the study of marker vaccine of CSFV C strain. In this study, CSFV C strain was used as the research material, and WH303 site was mutated by overlapping PCR method, and then cs-full and CS-full-flag were cloned into the full-length infectious clones of CSFV C strain. In vitro transcription RNA, and then transfected into PK15 cells, continuous passage of cells to complete the cell rescue and proliferation. The results showed that the specific restriction endonuclease digestion reaction verified the successful construction of infectious clone. RT-PCR and peroxidase monolayer cell test detection indicated that the virus had been rescued successfully. The virus can still be detected in the 17th generation of virus cell, indicating that the virus can be stably passaged. In summary, MC and MC-flag of C strain WH303 mutated infectious clones were constructed successfully in this study, MC strain was saved successfully, and the stable virus strain was obtained.

Key words: swine fever virus, C strain, site WH303, mutation, infectious clone

CLC Number:

S852.651

LIU Yuanjie, XU Lu, ZHU Yuanyuan, XU Yuan, ZHANG Qianyi, LI Cui, LI Ming, XIA Yingju, WANG Qin, LIU Yebing, ZHAO Qizu, ZOU Xingqi. The Construction and Rescue of Epitope Mutant Strain of Classical Swine Fever Virus C Strain[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 698-705.

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The Construction and Rescue of Epitope Mutant Strain of Classical Swine Fever Virus C Strain

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