原核表达的褐黄血蜱唾液腺蛋白和铁蛋白1的免疫保护效果评价

doi:10.11843/j.issn.0366-6964.2024.02.026

Abstract

Abstract: This study aimed to obtain rHfFer1 and rHfAV422 of Haemaphysalis flava and evaluate their anti-tick vaccine efficacy. The rHfFer1 and rHfAV422 recombinant proteins of H. flava were obtained by prokaryotic expression and twenty-two rabbits were randomly divided into three groups (Quil-A saponin control group and two immunization groups), among them, there were six rabbits in the Quil-A saponin control group and eight rabbits in each of the two immunized groups. Fourteen days after the first immunization, the same dose was given for the second immunization, and the larvae infestation were conducted one week after the second immunization. Immune efficacy was evaluated according to the engorged and molting rate. The results showed that the engorged and molting rate of the rHfFer1 and rHfAV422 immunized groups were significantly different from those of the control group (P<0.05), the calculated anti-tick vaccine efficacy of rHfFer1 and rHfAV422 were 68.06% and 50.00%, respectively. The result indicated that rHfFer1 and rHfAV422 could be used as candidate antigens for the development of anti-tick vaccines.

Key words: Haemaphysalis flava, ferritin 1, Fer1, tick salivary protein, AV422, recombinant proteins, anti-tick efficacy

CLC Number:

S852.746

CHEN Ling, CHEN Hao, YUE Chanjuan, MA Rui, FAN Xueyang, LIU Songrui, YANG Guangyou. Evaluation of the Immune Protection Effect of Prokaryotic Expressed Salivary Protein and Ferritin1 in Haemaphysalis flava[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 688-697.

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Evaluation of the Immune Protection Effect of Prokaryotic Expressed Salivary Protein and Ferritin1 in Haemaphysalis flava

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