利用牛乳腺细胞和小鼠乳腺组织分析异绿原酸C通过NF-κB信号通路对乳腺炎症反应的抑制效应

doi:10.11843/j.issn.0366-6964.2023.09.031

Abstract

Abstract: The research aims to investigate the inhibitory effect of isochlorogenic acid C (ICAC) on mastitis and NF-κB inflammatory pathway. In this study, bovine mammary epithelial cells (MAC-T) and mouse mammary tissue stimulated with lipopolysaccharide (LPS) were used as in vitro and in vivo inflammatory model, respectively. MTT method was used to screen the optimal concentrations of ICAC for MAC-T cell treatment. The expression levels of inflammatory factors (IL-1β, IL-6 and TNF-α) and inflammatory mediators (COX-2 and iNOS) were detected using ELISA, whereas the expression and phosphorylation levels of key proteins (IκB and p65) in NF-κB signaling pathway were detected by Western blot. The results showed that:1) ICAC did not significantly inhibit the growth of MAC-T cells at the concentrations from 20 to 100 mg·L^-1 (P>0.05); 2) The expression levels of IL-1β and IL-6 protein in MAC-T cells treated with 1 mg·L^-1 LPS were significantly higher than those in untreated group (P<0.01), but ICAC (20, 50, 80 mg·L^-1) significantly down-regulated the expression of IL-1β, IL-6, TNF-α, COX-2 and iNOS in MAC-T cells (P<0.01); 3) Intraperitoneal inject ICAC(60、80、100 mg·kg^-1) reduced LPS-induced CD3 expression and T lymphocyte infiltration in mammary tissues of mice; 4) ICAC significantly reduced the phosphorylation of p65 and IκB in LPS-induced MAC-T cells and in mouse mammary tissue (P<0.05), and it inhibited the nuclear transfer of p65. These results indicate that ICAC inhibits the inflammatory response in MAC-T cells and mouse mammary tissue through NF-κB inflammatory pathway, but the role of ICAC in the prevention and treatment of bovine mastitis needs further investigation.

Key words: isochlorogenic acid C, anti-inflammatory, NF-κB, MAC-T, mouse mammary gland

CLC Number:

S858.23

CHEN Xihong, LU Guicong, WANG Haolei, GOU Shaoxiao, YU Yongxiong, LIN Tao, JIANG Caode. Isochlorogenic Acid C Inhibits Mammary Inflammatory Response through NF-κB Signaling Pathway Using Bovine Mammary Gland Cells and Mouse Mammary Gland Tissue[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3931-3940.

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Isochlorogenic Acid C Inhibits Mammary Inflammatory Response through NF-κB Signaling Pathway Using Bovine Mammary Gland Cells and Mouse Mammary Gland Tissue

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