异体移植炎症因子1通过核因子κB信号促进牛乳腺上皮细胞炎症因子的释放

doi:10.11843/j.issn.0366-6964.2021.03.022

Abstract

Abstract: Bovine mastitis is the inflammatory response in bovine mammary gland, it is the most prevalent disease in dairy cattle worldwide. Many cytokines are inflammatory mediators. But the key cytokines associated with bovine mastitis have not been elucidated. Allograft inflammatory factor-1 (AIF-1) plays a central role in immune regulation and it is over-expressed in a vast array of inflammatory diseases. Therefore, we explored the possible role of bovine AIF-1 related to bovine mastitis in the present study. First, bovine AIF-1 gene was cloned by RT-PCR and the AIF-1protein was purified by a Ni-NTA spin column. Then, bovine mammary gland epithelial cell MAC-T line was treated with AIF-1 for 48 h. The concentrations of TNF-α, IL-6, AIF-1 and monocyte chemoattractant protein 1 secretion were measured by ELISA assay kits. Finally, phosphorylation of IκBα was detected by Western blot. The results showed that the average concentration of AIF-1 in milks suffering from mastitis was significantly higher than that in the healthy cows, while its value decreased in cows recovered from mastitis. Furthermore, bovine AIF-1 gene was cloned and recombinant bovine AIF-1 protein was prepared. The protein up-regulated TNF-α, IL-6 and monocyte chemoattractant protein 1 secretion from bovine mammary epithelial cells with NF-κB activating, then NF-κB signaling inhibitor BAY 11-7085 abolished the increase of these inflammatory cytokine secretion induced by AIF-1. The present study indicated that AIF-1 induced inflammatory mediator production from bovine mammary epithelial cells via NF-κB signaling.

Key words: bovine mastitis, bovine mammary epithelial cells, allograft inflammatory factor-1, NF-κB

CLC Number:

S857.26

WU Yinghuan, YANG Danru, ZHAO Yanying. Bovine Allograft Inflammatory Factor-1 (AIF-1) Induces Inflammatory Mediator Secretion from Bovine Mammary Epithelial Cells via NF-κB Signaling[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(3): 782-788.

References 31

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Bovine Allograft Inflammatory Factor-1 (AIF-1) Induces Inflammatory Mediator Secretion from Bovine Mammary Epithelial Cells via NF-κB Signaling

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