苦参碱联合不同抗生素抗炎作用比较研究

doi:10.11843/j.issn.0366-6964.2023.10.037

Abstract

Abstract: The aim of this study was to compare the anti-inflammatory effects of matrine combined with different antibiotics. Five antibiotics including amoxicillin, chlortetracycline, doxycycline hydrochloride, florfenicol and tilmicosin were selected in this experiment. Using porcine reproductive and respiratory syndrome virus (PRRSV) 5'UTR RNA and lipopolysaccharide (LPS) co-stimulated porcine alveolar macrophage (PAMs) as inflammatory model, IL-1β as the detection index, to screen antibiotics with similar anti-inflammatory effect to matrine. Then using qPCR and Western blot to detect the effect of matrine combined with antibiotics on IL-1β expression. The results showed that, the inhibitory effect of amoxicillin and aureomycin treatment group were consistent with that of matrine on IL-1β mRNA expression. When matrine was used in combination with amoxicillin or aureomycin, an interaction was noted between matrine and amoxicillin, while matrine and aureomycin lack such type of interation, which indicated that matrine could minimize part dosage of amoxicillin. These results indicated that, in the process of inhibiting IL-1β expression induced by PRRSV 5'UTR RNA and LPS co-stimulation, when combined with amoxicillin, matrine can partially replace the efficacy of amoxicillin and reduce the dosage of amoxicillin. This study provides data support for the clinical application of matrine as an antibiotic substitute.

Key words: matrine, antibiotic, amoxicillin, antibiotic substitution, anti-inflammatory

CLC Number:

S859.7

SUN Panpan, CAO Zhigang, LING Xiaoya, SUN Na, SUN Yaogui, LI Hongquan. Comparison of Anti-inflammatory Effects of Matrine Combined with Different Antibiotics[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4411-4421.

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Comparison of Anti-inflammatory Effects of Matrine Combined with Different Antibiotics

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