高聚合度菊粉通过调节肠-脂肪组织轴改善高脂饮食诱导的犬肥胖

doi:10.11843/j.issn.0366-6964.2023.09.032

Abstract

Abstract: The aim of this study was to investigate the mechanism of inulin with high degree of polymerization in improving high-fat diet-induced obesity in dogs based on the gut-adipose tissue axis. Forty poodle were randomly divided into five groups:common diet group, high-fat diet group, and low-dose, medium-dose and high-dose inulin with high degree of polymerization groups. Dogs in common diet group were fed with normal diet, and in high-fat diet were fed with high-fat diet. Dogs in low-dose, medium-dose and high-dose inulin with high degree of polymerization groups were fed with high-fat diet containing 1.0%, 3.0% and 5.0% inulin with high degree of polymerization, respectively. The test period was 12 weeks. After the experiment, the levels of serum glucose, fat and inflammatory factors were measured, and the relative expressions of mRNA and protein of related factors in subcutaneous adipose tissue and colon mucosa were detected using real-time fluorescent PCR and Western blot, respectively. The results showed as follows:Inulin with high degree of polymerization effectively reduced the body weight, body fat rate and blood lipid level, meanwhile improved the impaired glucose tolerance in dogs fed with high-fat diet. Moreover, inulin with high degree of polymerization increased the mRNA and protein expression of Occludin and ZO-1 in colonic mucosa, and reduced levels of serum lipopolysaccharide (LPS), IL-6 and TNF-ɑ and the expression of IL-6 and TNF-ɑ in adipose tissue. In conclusion, inulin with high degree of polymerization can improve the obesity induced by high-fat diet in dogs, and its mechanism may be related to the regulation of the gut-adipose tissue axis of "intestinal mucosal barrier function-LPS translocation-adipose tissue inflammation".

Key words: inulin with high degree of polymerization, high-fat diet, dog, obesity, gut-adipose tissue axis

CLC Number:

S856
S858.29

LU Jiang, ZHU Daoxian, LU Jinye, LIU Li, HAO Fuxing, WU Zhi, LU Wei, LIU Jing. Inulin with High Degree of Polymerization Improves High-fat Diet Induced Obesity in Dogs by Regulating the Gut-adipose Tissue Axis[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3941-3950.

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Inulin with High Degree of Polymerization Improves High-fat Diet Induced Obesity in Dogs by Regulating the Gut-adipose Tissue Axis

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