痛风雏鹅肠道菌群通过TLR4/MyD88/NF-κB通路促进肾损伤的研究

doi:10.11843/j.issn.0366-6964.2021.02.027

Abstract

Abstract: This study aimed to investigate the effect of gut microbiota on renal injury and its mechanism in gout gosling. In this study, 15 goslings with typical gout characteristics and 15 healthy goslings of the same age and growth environment were selected. 16S rDNA sequencing technology was used to analyze the difference of bacterial diversity in cecal contents. Serum creatinine (Cr), uric acid (UA) and urea nitrogen (BUN) were respectively measured by modified picric acid method, enzyme colorimetric method and urease glutamate dehydrogenase method. RT qPCR and Western blot were respectively used to detect the mRNA and protein expressions of toll-like receptor 4 (TLR4), myeloid differentiation factor (MyD88), nuclear transcription factor-κB (NF-κB), interleukin-1β (IL-1β), tumor necrosis factor-ɑ (TNF-ɑ) and interleukin-6 (IL-6). Lastly, the gut microbiota was transplanted into sterile mice for the verification test. The results showed that: 1) Compared with healthy goslings, the number of operational taxonomic units (OTUs), Chao 1 index and Shannon index of gut microbiota in gout goslings were significantly decreased (P<0.01), and the abundance of Proteobacteria, Escherichia, Proteus and Enterococcus increased significantly. The gut microbiota of gout goslings had higher participation in the immune system, bacterial infection, membrane transport and nucleotide metabolism. 2) Compared with healthy goslings, the mRNA and protein expressions of TLR4, MyD88, NF-κB, IL-1β, TNF-α and IL-6 in kidney tissue of gout goslings were significantly increased (P<0.05 or 0.01). The mRNA expression levels of these molecules were positively correlated with Cr, BUN and UA, and strongly positively correlated with the abundance of Escherichia, Proteus and Enterococcus. 3) The mRNA expression of TLR4, MyD88, NF-κB in kidney tissue and the levels of Cr, BUN and KIM-1 in blood of mice transplanted gut microbiota of gout gosling were significantly higher than those of mice transplanted gut microbiota of healthy gosling. These changes could be reversed by TAK-242. In summary, gut microbiota changed in gout gosling can activate LTR4/MyD88/NF-κB signaling pathway, induce inflammatory response, and promote kidney injury.

Key words: gout gosling, gut bacteria, 16S rDRNA sequencing, LTR4/MyD88/NF-κB signaling pathway, kidney injury

CLC Number:

S856.5

ZHU Daoxian, WU Zhi, LU Jiang, HUANG Tao, LIU Li. Gut Bacteria of Gout Gosling Promoted Kidney Injury Through LTR4/MyD88/NF-κB Signaling Pathway[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(2): 543-552.

References 23

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Gut Bacteria of Gout Gosling Promoted Kidney Injury Through LTR4/MyD88/NF-κB Signaling Pathway

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