蒙古马和纯血马免疫相关基因差异表达分析

doi:10.11843/j.issn.0366-6964.2023.12.013

Abstract

Abstract: The purposes of this study were to evaluate the variations in gene expression between Mongolian horse and Thoroughbred, which underlying the coordinate changes in the immunophysiological responses to the specific environmental conditions and directional breeding selection pressure. In this study, jugular blood collected from healthy adult Mongolian horses (n=3) and Thoroughbred (n=3) was used to perform RNA-Seq and comparative analysis of the expression profiles between two horse breeds. Liver, spleen, lung, and jugular blood samples were collected from about 2-year-old healthy Mongolian horses (n=3) and Thoroughbred (n=3). qRT-PCR was used to analyze the relative expression of 7 genes of NF-κB signaling pathway in liver, spleen, lung and blood tissues between two horse breeds. Western blot was used to analyze the relative expression of RELA proto-oncogene NF-kB subunit (NF-κB p65) protein and interleukin 1 beta (IL-1β) protein in the lungs between Mongolian horse and Thoroughbred. The results showed that 19.69-23.95 Gb high-quality RNA-Seq data were obtained and 1 199 differentially expressed genes were identified. Functional analysis showed that many differentially expressed genes directly participated in the immune process. The qRT-PCR results showed that 7 genes of NF-κB signaling pathway were expressed in 4 tissues of Mongolian horse and Thoroughbred, but the difference in gene expression between breeds was manifested as tissue diversity. The expression of 7 genes in the lung of Mongolian horse was higher than that of Thoroughbred, excepting for tumor necrosis factor (TNF-α) (P≥0.05), while all other genes had biological statistical significance (P<0.05). The expression of NF-κB p65 protein and IL-1β protein in the lung of Mongolian horse was also higher than that of Thoroughbred (P<0.05). Many immune-related genes were differentially expressed in the immune tissues between Mongolian horses and Thoroughbred, which might be due to the adaptation of Mongolian horses to harsh environments and the sports performance directional breeding of Thoroughbred. The results of this study provided data support for further research on the molecular mechanism of the Mongolian horse immune function adapting to specific environment.

Key words: Mongolian horse, Thoroughbred, immune-related gene, NF-κB signaling pathway

CLC Number:

S821.2

REN Xiujuan, KANG Decuo, SU Shaofeng, LIN Yanan, LI Yajing, DU Ming, BAI Dongyi, LI Bei, ZHAO Yiping, DUGARJAVIIN Manglai. Comparison of Immune Gene Expression between Mongolian Horse and Thoroughbred[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5020-5032.

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Comparison of Immune Gene Expression between Mongolian Horse and Thoroughbred

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