猪瘟病毒非结构蛋白NS5A与Beclin1相互作用并促进病毒增殖

doi:10.11843/j.issn.0366-6964.2023.02.028

Abstract

Abstract: In order to explore the role of host protein Beclin1 in the process of autophagy activated by NS5A, a non structural protein of classical swine fever virus (CSFV), and the specific molecular mechanism, this study used qRT-PCR to detect the expression changes of Beclin1, PI3K/Akt signaling pathway related factors in ST cells infected with CSFV and expressing NS5A protein. The interaction between Beclin1 and NS5A was studied by laser confocal, Co IP and GST pulldown methods. By overexpressing or knocking down Beclin1 in ST cells, we studied its effect on CSFV replication. The results showed that ST cells infected with CSFV or expressed NS5A protein externally, the transcription and protein expression levels of Beclin1 were significantly increased, and the expression levels of PI3K/Akt signaling pathway related factors were positively correlated with them. In addition, CSFV NS5A protein and Beclin1 protein have co localization and interaction in cells. Finally, we found that overexpression of Beclin1 in cells significantly promoted CSFV replication; On the contrary, knockdown of Beclin1 with siRNA inhibited the activation of PI3K/Akt signaling pathway, and CSFV proliferation showed significant inhibitory effect. The above results indicate that Beclin1 protein can promote the replication of CSFV, and its mechanism is through the interaction with NS5A to regulate PI3K/Akt signaling pathway.

Key words: PI3K/Akt signaling pathway, CSFV, Beclin1, protein interaction, autophagy

CLC Number:

S852.659.6

ZHANG Chengcheng, SUN Jiahao, WANG Xiuling, ZHANG Xiaorong, WU Yantao. Beclin1 Interacts with the Nonstructural Protein NS5A of CSFV and Promotes Virus Proliferation[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 715-725.

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Beclin1 Interacts with the Nonstructural Protein NS5A of CSFV and Promotes Virus Proliferation

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