宫内发育迟缓对环江香猪哺乳仔猪IGF发育模式的影响

doi:10.11843/j.issn.0366-6964.2023.06.020

Abstract

Abstract: This study was conducted to investigate the relationship between the occurrence of intrauterine growth retardation (IUGR) and the development pattern of insulin-like growth factor (IGF). Twenty pregnant sows with similar body condition were selected. The largest piglet selected was defined as normal birth weight (NBW) piglet and the smallest piglet was defined as IUGR piglet from each litter after parturition. They were divided into NBW group and IUGR group with 20 piglets per group. At 0, 7, 14, and 21 days of age, 5 piglets were randomly selected from each group, respectively. The blood was collected from the anterior vena cava and the plasma was collected to detect the IGF-1 concentration, and the samples of liver and longissimus dorsi muscle were collected to detect the gene expression levels of IGF-1, IGF-1R,IGFBP-3, and IGFBP-5. The results showed that the body weight and plasma IGF-1 content of IUGR piglets were significantly lower (P＜0.05) during 0 to 21 days of age, compared with the NBW piglets. The body weight of IUGR piglets during 0 to 14 days of age was increased (P＜0.05), but there were no significant difference (P＞0.05) in the body weight of IUGR piglets at 14 and 21 days of age. At 0 day of age, the IUGR piglets had significantly higher (P＜0.05) mRNA expressions of IGF-1R, IGFBP-3 and IGFBP-5 in muscle and IGFBP-5 in liver than NBW piglets; At 7 days of age, the IUGR piglets had significantly lower (P＜0.05) mRNA expression levels of IGF-1 in muscle or liver than NBW piglets; At 21 days of age, the IUGR piglets had significantly higher (P＜0.05) mRNA expression levels of IGF-1R in muscle than NBW piglets. The expression of IGF-1 in liver and muscle of NBW piglets and liver of IUGR piglets at 7 days of age was significantly higher (P＜0.05) than that at other days of age; The mRNA expression level of IGF-1 in muscle of IUGR piglets at 0 and 14 days of age was significantly higher (P＜0.05) than that of IUGR piglets at 7 and 21 days of age. The expression level of IGFBP-5 in liver and muscle of NBW piglets at 7 days of age was significantly higher (P＜0.05) than that at other days of age, and the expression levels of IGFBP-3 at 14 days of age and IGFBP-5 at 0 day of age were significantly up-regulated (P＜0.05) in liver of IUGR piglets. Collectively, these findings suggested that the IUGR may reduce the expression of IGF-1 and regulate the expression of IGF-1 receptor and binding protein in suckling piglets of Huanjiang mini-pig, which may lead to the decrease of IGF-1 content, retard growth and development of suckling piglets.

Key words: intrauterine growth retardation, Huanjiang mini-pig, insulin-like growth factor, receptor, binding protein

CLC Number:

S828.4

GENG Meimei, DOU Mengying, FU Dezhi, HE Qinghua, KONG Xiangfeng. Effects of Intrauterine Growth Retardation on Developmental Model of Insulin-Like Growth Factor in Suckling Piglets of Huanjiang Mini-Pig[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2414-2420.

References 26

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Effects of Intrauterine Growth Retardation on Developmental Model of Insulin-Like Growth Factor in Suckling Piglets of Huanjiang Mini-Pig

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