甲硫脑啡肽通过阿片受体促进脂多糖作用下奶牛子宫内膜基质细胞的增殖

doi:10.11843/j.issn.0366-6964.2023.03.034

Abstract

Abstract: To clarify the mechanism of Met-enkephalin (MENK) on the proliferation of bovine endometrial stromal cell (BESC), the BESC were co-treated with lipopolysaccharide (LPS), opioid receptor antagonists and MENK. The opioid receptor antagonists used in this study included the nonselective opioid receptor antagonist naloxone (Nx), the δ opioid receptor antagonist ICI 154129 were the μ opioid receptor antagonist CTAP, and the groups were as follows: the blank control group, the LPS treatment group, the LPS and MENK (10^-10 mol·L^-1) co-treatment group (LM group), the LPS, MENK and opioid receptor antagonists (Nx, CTAP and ICI 154129 were all 10^-10 mol·L^-1) co-treatment group and the LPS and opioid receptor antagonist co-treatment groups. Cell viability, cell cycle, the expression of cell proliferation-related genes and the expression of Wnt/β-catenin and PI3K/AK pathway proteins were detected. The results showed that the treatment of opioid receptor antagonist alone in treatment concentration or co-treatment of LPS for 24 h had no effect on cell viability (P>0.05). Compared with the LM group, the cell numbers of G1 phase increased (P<0.05) in the co-treatment groups of LPS, MENK and Nx (LMN) and LPS, MENK and ICI 154129 (LMI), the cell numbers of S phase decreased (P<0.05) in LMN group and the co-treatment group of LPS, MENK and CTAP (LMC). Compared with LM group, the gene expressions of CCN2, TGFB1 and TGFB3 decreased (P<0.01) in the groups of LMN and LMI; the CCN2 expression decreased (P<0.01) in LMC group. Compared with LM group, there were increases in the protein expressions of cyclinD1 and GSK-3β in LMN group (P<0.05), the β-catenin and GSK-3β in LMC group (P<0.01) , and the cyclinD1 in LMC group (P<0.05) . Compared with LM group, the phosphorylation level of AKT increased (P<0.05) in LMN, LMI, and LMC groups. In conclusion, both δ opioid receptor and μ opioid receptor are involved in the proliferation of MENK on BESC.

Key words: Met-enkephalin, bovine endometrial stromal cell, opioid receptors, opioid receptor antagonist, proliferation

CLC Number:

S857.21

SUN Wenye, LI Jianji, WANG Heng, DONG Junsheng, LI Jun, CUI Luying. Effect of Met-enkephalin on Lipopolysaccharide-stimulated Proliferation of Bovine Endometrial Stromal Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1229-1239.

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Effect of Met-enkephalin on Lipopolysaccharide-stimulated Proliferation of Bovine Endometrial Stromal Cells

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