反刍动物Toll样受体多基因家族的分子进化及表达模式分析

doi:10.11843/j.issn.0366-6964.2023.09.013

Abstract

Abstract: The study aimed to understand the molecular evolutionary relationships and expression analysis of the multigene family of Toll-like receptors (TLRs) in ruminants. The TLR1-10 genes was systematically identified in ruminants based on the genomes of 53 ruminant species from 6 families, their chromosomal localization and gene structure, evolutionary relationships, selection pressure (ω) was analyzed, and the expression patterns of TLR genes was clarified combined with transcriptomic data in various tissues. The results showed that:1) TLR1-10 in ruminants were all single-copy genes, which were classified into TLR1 (TLR1/6/10), TLR2, TLR4, TLR3 (TLR3/5) and TLR7 (TLR7/8/9) subfamilies according to evolutionary relationships. 2) The 10 TLR genes in ruminants as a whole underwent purifying selection (ω<1), but a total of 35 amino acid loci were subject to strong positive selection; bovidae had higher ω values and a higher proportion of positive selection loci than cervidae, and non-viral TLRs had higher ω and more positive selection loci than viral TLRs, suggesting that cervidae TLRs as well as viral TLRs were subject to stronger evolutionary binding. 3) In sheep, all 10 TLR genes were expressed in immune tissues, with TLR2 and TLR9 expressed at relatively high levels in PBMC. The results suggest that the gene family of ruminant TLRs is relatively conserved at the evolutionary level and both are subject to strong purifying selection, with TLR2 and TLR9 playing a major immune role in PBMC tissues.

Key words: Toll-like receptor, ruminant, phylogeny, selection pressure, expression pattern

CLC Number:

S813.2

MA Shujuan, XU Yijie, HE Ke, MA Ruifeng, ZHU Ying. Molecular Evolution and Expression Patterns of a Multigene Family of Toll-like Receptors in Ruminants[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3722-3734.

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Molecular Evolution and Expression Patterns of a Multigene Family of Toll-like Receptors in Ruminants

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