miR-144-5p靶向WNT5a对山羊卵巢颗粒细胞增殖、凋亡的影响

doi:10.11843/j.issn.0366-6964.2023.06.021

Abstract

Abstract: The study aimed to investigate the effect of miR-144-5p targeting WNT5a on proliferation and apoptosis of goat granulosa cells. Ovarian granulosa cells from 2-year-old healthy Leizhou female goats were selected to overexpress and repress miR-144-5p and WNT5a, and the effects of miR-144-5p and WNT5a on granulosa cell proliferation and apoptosis were investigated by fluorescent quantitative PCR (real-time qPCR, RT-qPCR), Western blot, dual luciferase reporter gene, CCK-8 and other techniques. The results showed that the expression of miR-144-5p was significantly higher in large follicular granulosa cells than that in small follicular granulosa cells (P<0.01), and the expression of WNT5a was significantly lower in large follicular granulosa cells than that in small follicular granulosa cells (P<0.01); CCK-8 assay revealed that miR-144-5p inhibited the proliferation of GCs, and overexpression of miR-144-5p resulted in significant downregulation of mRNA and protein expression of the apoptosis suppressor gene BCL-2 (P<0.01) and significant upregulation of mRNA and protein expression of the pro-apoptotic gene BAX (P<0.01); Wnt/β-Catenin pathway gene CTNNB1 mRNA and β-Catenin protein levels were highly significantly reduced (P<0.01); In addition, miR-144-5p could target WNT5a to inhibit its expression. Meanwhile, CCK-8 assay results showed that WNT5a promoted the proliferation of GCs, and overexpression of WNT5a significantly upregulated the mRNA and protein levels of the BCL-2 (P<0.01), significantly downregulated the mRNA level (P<0.05) and protein level (P<0.01) of BAX, and the Wnt/β-Catenin pathway gene CTNNB1 mRNA and β-Catenin protein levels were significantly increased (P<0.01). In summary, in GCs, miR-144-5p targets and negatively regulates WNT5a expression, regulates the Wnt/β-Catenin pathway, and then upregulates the BAX/BCL-2 ratio to inhibit granulosa cell proliferation and promote apoptosis, which may have an effect on ovulation and lambing numbers in goats.

Key words: granulosa cells, miR-144-5p, WNT5a, goat, reproduction

CLC Number:

S827.3

LIU Jie, XU Xiangping, DENG Ming, ZOU Xian, JIANG Shengwei, LIU Dewu, LIU Guangbin, SUN Baoli, GUO Yongqing, LI Yaokun. Effect of miR-144-5p Targeting WNT5a on the Proliferation and Apoptosis of Goat Ovarian Granulosa Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2421-2435.

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Effect of miR-144-5p Targeting WNT5a on the Proliferation and Apoptosis of Goat Ovarian Granulosa Cells

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