雄激素受体抑制剂恩杂鲁胺对山羊卵泡颗粒细胞增殖凋亡的影响

doi:10.11843/j.issn.0366-6964.2024.05.020

Abstract

Abstract: The aim of this study was to investigate the effects of androgen receptor (AR) on goat granulosa cell proliferation and apoptosis using enzalutamide. Firstly, granulosa cells from goats were isolated and subjected to in vitro culture. The suitable concentration of enzalutamide for cell culture was determined by microscopic observation and CCK8 assay. The experiment was then divided into 3 groups: blank control group (no treatment), negative control group (treated with DMSO), and experimental group (treated with enzalutamide). Each group was set up with 3 replicates. Cell proliferation was detected using the EDU assay, while cell apoptosis was assessed using Caspase3 activity and apoptosis detection kits. The expression of genes and proteins related to cell proliferation and apoptosis was evaluated using quantitative real-time PCR (qRT-PCR) and Western blot analysis. The results showed that the AR inhibitor, enzalutamide, significantly inhibited cell proliferation and reduced the mRNA levels of CCND1, PCNA, and MKI67 in granulosa cells. It also suppressed the protein expression of CCND1, PCNA, and β-catenin. Additionally, enzalutamide promoted apoptosis in goat granulosa cells, as evidenced by increased mRNA levels of BAX and BCL2, without affecting their protein expression. Furthermore, enzalutamide, while not affecting the mRNA level of CASP3, enhanced Caspase3 activity. These findings suggest that enzalutamide, by inhibiting AR activity, can modulate the expression of β-catenin, PCNA, CCND1, Caspase 3, and other genes involved in the regulation of granulosa cell proliferation and apoptosis.

Key words: goat, granulosa cell, androgen receptor, enzalutamide, proliferation apoptosis

CLC Number:

S827.3

DONG Shucan, MAO Shuaixiang, WU Cuiying, LI Yaokun, SUN Baoli, GUO Yongqing, DENG Ming, LIU Dewu, LIU Guangbin. The Effect of the Androgen Receptor Inhibitor Enzalutamide on Proliferation and Apoptosis of Goat Ovarian Granulosa Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2022-2031.

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The Effect of the Androgen Receptor Inhibitor Enzalutamide on Proliferation and Apoptosis of Goat Ovarian Granulosa Cells

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