绵羊生长性状间的相关和回归分析及TRHDE基因多态性与生长性状的关联分析

doi:10.11843/j.issn.0366-6964.2023.04.007

Abstract

Abstract: This study aimed to explore the correlation among growth traits in Ujumqin sheep and the optimal regression equation of body size to body weight was established, and analyze the effect of TRHDE gene polymorphism on the growth traits of Ujumqin sheep. In this study, 336 well-developed Ujumqin sheep (176 males and 160 females) were selected as research objects, and growth traits were collected at 4-month-old and 6-month-old. Genotypes of SNPs in the TRHDE gene were detected using the sheep Illumina OvineSNP600 BeadChip in a population of 336 Ujumqin sheep. SPSS 26.0 software was used for correlation and regression analysis among growth traits and association analysis between TRHDE gene polymorphism and growth traits. The results showed that the optimal regression equations of 4-month-old and 6-month-old male and female Ujumqin sheep were as follows:Y₁=-53.66+0.77X₁+0.39X₂, Y₂=-47.83+0.63X₁+0.45X₂, Y₃=-47.01+0.42X₁+0.13X₂+2.82X₃+0.19X₄+0.28X₅, Y₄=-61.32+0.25X₁+0.40X₂+3.15X₃+0.39X₄. The results of the association of TRHDE gene SNPs with growth traits in Ujumqin sheep showed that rs404402551A>G locus was significantly or extremely significantly associated with body weight and chest circumference at 4-month-old and body weight, chest circumference and chest width at 6-month-old (P<0.05; P<0.01). The rs415608385C>A locus was significantly or extremely significantly associated with body weight at 4-month-old and body weight, body height, body length and chest width at 6-month-old (P<0.05; P<0.01). The rs426657887G>A locus was not significantly associated with each growth trait in Ujumqin sheep (P>0.05). And the rs404402551A>G and rs426657887G>A loci were in strong linkage disequilibrium in the Ujumqin sheep. In conclusion, the greater potential for selection of body weight in Ujumqin sheep and chest circumference can be used as an important indicator for assessing body weight in Ujumqin sheep. The rs404402551A>G and rs415608385C>A loci of the TRHDE gene can be used as potential molecular markers for growth and development in Ujumqin sheep, which can provide reference for molecular marker-assisted selection for breeding in Ujumqin sheep.

Key words: Ujumqin sheep, TRHDE gene, growth traits, correlation analysis, regression analysis, association analysis

CLC Number:

S826.2

BI Yazhen, SHANG Mingyu, HU Wenping, ZHANG Li. Correlation and Regression Analysis among Growth Traits and Association Analysis of TRHDE Gene Polymorphism with Growth Traits in Sheep[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1415-1428.

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Correlation and Regression Analysis among Growth Traits and Association Analysis of TRHDE Gene Polymorphism with Growth Traits in Sheep

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