北京黑猪FABP3和SCD基因多态性与肉品质性状关联分析

doi:10.11843/j.issn.0366-6964.2023.03.011

Abstract

Abstract: The study aimed to investigate the polymorphism of fatty acid binding protein 3 (FABP3) and stearoyl-CoA desaturase (SCD) genes and their association with meat quality traits. It can guide the breeding of Beijing black pigs at molecular level. In this study, meat quality and trait data of 413 Beijing black pigs were collected, DNA and RNA was extracted. According to FABP3 and SCD gene sequences, 20 pairs of primers were designed for PCR amplification and sequencing. DNAStar software was used to analyze the sequencing results. The correlation between FABP3 and SCD genotypes and intramuscular fat content of Beijing black pigs was analyzed and the gene expression difference was analyzed by fluorescence quantitative PCR. FABP3 gene was identified a missense mutation (c. 681 A>G), SCD gene was identified a missense mutation, two synonymous mutations and two 3'UTR region mutations. Duncan’s multiple test statistical analysis showed that all SNPs in SCD gene were not significantly associated with meat quality traits. FABP3 missense mutant c.681A>G was significantly associated with carnation L^* and intramuscular fat (IMF) content (P<0.05). The missense mutation resulted in the mutation of amino acid residue 51 of FABP3 from isoleucine (I) to threonine (T). qPCR was used to analyze the differences in individual expression levels of two homozygous genotypes of c.681A>G (AA and GG) of FABP3, and it was found that there was no significant difference in gene expression levels between the individuals with two homozygous genotypes at the mutation site. These results suggested that the missense mutation site of FABP3 c.681A>G may regulate the intramuscular fat content and carnation L^* traits by affecting the protein function. Only one locus of the FABP3 and SCD genes was significantly associated with carnation L^* and intramuscular fat content, which could be used as a candidate gene functional locus for meat quality traits of Beijing black pigs and also provide a new molecular marker for meat quality breeding of Beijing black pigs.

Key words: Beijing black pigs, FABP3, SCD, meat quality, association analysis

CLC Number:

S828.2

SU Yanfang, YANG Man, NIU Naiqi, HOU Xinhua, ZHANG Longchao. Association Analysis of FABP3 and SCD Gene Polymorphisms with Meat Quality Traits in Beijing Black Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 966-975.

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Association Analysis of FABP3 and SCD Gene Polymorphisms with Meat Quality Traits in Beijing Black Pigs

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