杜洛克猪生长性状全基因组关联分析及候选基因鉴定

doi:10.11843/j.issn.0366-6964.2023.05.010

Abstract

Abstract: This study aimed to perform genome-wide association study for candidate gene identification of growth traits in Duroc pigs. In this study, 361 Duroc boars were selected as the experimental population, the days to 100 kg, average daily gain to 100 kg, average backfat thickness to 100 kg and loin muscle area to 100 kg traits were measured. The genotype information was typed by 50K single nucleotide polymorphism array, and 31 618 SNPs were obtained after quality control. The genetic parameters of growth traits were estimated using genome information by GCTA software, and candidate genes related to growth traits were identified by GWAS using R software rMVP package FarmCPU model. The results showed that the heritabilities of the days to 100 kg, average daily gain to 100 kg, average backfat thickness to 100 kg and loin muscle area to 100 kg were 0.27, 0.29, 0.16 and 0.11, respectively, which were moderate heritability traits. The genetic correlation and phenotypic correlation of days to 100 kg and average daily gain to 100 kg were -0.99, indicating the strong negative correlation. The results of GWAS showed that 3 significant SNPs were detected on the days to 100 kg and average daily gain to 100 kg traits, located on chromosome 10. Multiple comparison of allelic genotypes of significant SNPs was performed by Least significant difference test. The dominant alleles of significant SNPs rs81237156, rs81424502 and rs81313018 were G, T, and A, respectively. Candidate genes HACD1 and BAMB were identified to be associated with pig growth traits in the candidate region near significant SNPs. In this study, the newly discovered candidate genes will promote the understanding of growth traits, and the identification of new variants can provide new insights for potential markers in pig breeding.

Key words: pig, growth traits, GWAS, candidate genes, FarmCPU

CLC Number:

S828.2

ZHANG Xiaoke, LIAO Weili, CHEN Xinyou, LI Tingting, YUAN Xiaolong, LI Jiaqi, HUANG Xiang, ZHANG Hao. Genome-wide Association Study for Identifying Candidate Genes of Growth Traits in Duroc Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1868-1876.

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Genome-wide Association Study for Identifying Candidate Genes of Growth Traits in Duroc Pigs

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