氧化应激状态下仔猪海马差异表达miRNAs与转录组的关联分析

doi:10.11843/j.issn.0366-6964.2021.05.025

Abstract

Abstract: This study aims to elucidate the target genes of microRNA (miRNA) involved in the oxidative stress reaction in the dorsal and ventral hippocampus of piglets and its possible regulatory mechanism. In the study, based on the data of miRNAs and transcriptome packets previously put into the gene expression database (GEO), the target genes of differentially expressed miRNAs in the dorsal and ventral regions of the hippocampus of Rongchang piglets under oxidative stress were predicted by bioinformatics analysis. The predicted results and transcriptome data were overlapped and screened to get the target pairs, which were used to construct a network map of miRNA-mRNA interaction. GO classification and KEGG analysis were performed to explore the molecular mechanisms involved in the regulation of oxidative stress in the dorsal and ventral regions of the hippocampus of piglets. The results showed that the oxidative stress-induced differential expression of 309 pairs of miRNAs-mRNAs in the dorsal hippocampus and 247 pairs in the ventral hippocampus of piglets. Among those, there were 67 pairs of negatively regulated miRNA-mRNAs in the dorsal hippocampus (2 pairs of up-regulated miRNA-down-regulated mRNAs, 65 pairs of down-regulated miRNA-up-regulated mRNAs), and 41 pairs in the ventral hippocampus (3 pairs of up-regulated miRNA-down-regulated mRNAs, 38 pairs of down-regulated miRNA-up-regulated mRNAs). GO classification and KEGG analysis results displayed that targeted up-regulated genes played a major role in regulating oxidative stress damage and maintaining body homeostasis, while targeted down-regulated genes in the dorsal hippocampus mainly regulated cell apoptosis and those in the ventral hippocampus mainly regulated cell proliferation and migration. And some differentially expressed miRNAs participated in the regulation of oxidative stress-related neurological diseases in a coordinated or divergent manner through targeting regulating genes. In conclusion, the study firstly described the interaction network and signaling pathway of oxidative stress-related miRNA-mRNAs in the hippocampus of piglets, and analyzed the differences and consistency in the regulation of oxidative stress in the dorsal and ventral hippocampus, which laid a foundation for the study of the pathological mechanism of oxidative stress-mediated neurological diseases.

Key words: oxidative stress, hippocampus, miRNA-mRNA, correlation analysis

CLC Number:

S852.16

ZHANG Chen, FENG Luqiu, LIN Hailan, HUANG Ziqing, WANG Naixiu, GAN Ling. Association Analysis of Differentially Expressed miRNAs and Transcriptomics in Piglet Hippocampus under Oxidative Stress[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(5): 1396-1406.

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Association Analysis of Differentially Expressed miRNAs and Transcriptomics in Piglet Hippocampus under Oxidative Stress

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